摘 要 目的: 探讨细胞外基质蛋白1(extracellular matrix protein 1,ECM1)在乳腺癌组织和细胞株中的表达及其意义。方法： 应用免疫组织化学和免疫细胞化学EnVision法检测乳腺癌手术切除标本中乳腺组织（正常组织和癌组织）和恶性程度不同的乳腺癌细胞株（MCF-7、Sk-Br-3和MDA-435）中ECM1的表达，并比较正常乳腺组织和乳腺癌组织、淋巴结转移和未转移乳腺癌之间的ECM1表达差异。Western blotting检测确定ECM1亚型蛋白。结果：ECM1的表达在正常乳腺组织中阳性率为8.7%(2/23)，在乳腺癌组织中阳性率为60.4%(29/48)；其中乳腺癌淋巴结未转移者为38.5% (10/26)，淋巴结转移者为86.4% (19/22)。统计分析表明，ECM1的表达在乳腺癌组织中高于正常组织(P<0.01)，在转移性乳腺癌中高于非转移性乳腺癌（P<0.01）。恶性程度低的乳腺癌细胞株MCF-7、Sk-Br-3中不表达ECM1，而恶性程度较高的MDA-435细胞株中高表达ECM1。乳腺癌细胞中高表达的ECM1蛋白主要是相对分子质量为68 000的ECM1a亚型。结论： ECM1（主要为ECM1a亚型）在乳腺癌组织和恶性程度高的乳腺癌细胞中过表达，并且与乳腺癌的淋巴结转移相关。

Abstract Objective: To investigate the expression of extracellular matrix protein 1 (ECM1) in breast cancer tissues and cell lines and to assess its significance. Methods: Immunohistochemical and immunocytochemical EnVision methods were used to detect expression of ECM1 in the breast tissues（normal tissues and cancer tissues） and in breast cancer cell lines of different malignancies ( MCF7，SkBr3，and MDA435). The ECM1 positive rates were compared between normal tissues and cancer tissues and between cancer tissues with and without lymph node metastasis. Western blotting was used to identify the subtypes of ECM1. Results: The positive rate of ECM1 was 8.7%(2/23) in the normal breast tissues, 60.4%(29/48) in breast cancer tissues, 38.5% (10/26) in breast cancer tissues without lymph node metastasis, and 864% (19/22) in those with lymph node metastasis. Statistical analysis indicated that the ECM1 expression was higher in the breast cancer tissues than in the normal tissues (P<0.01), and the expression in the metastatic breast cancer was higher than that in the primary cancer tissues (P<0.01). Cell lines with low malignancy, such as MCF7 and SkBr3 cells, were negative of ECM1; cell lines with high malignancy, such as MDA435 cells, were strongly positive of ECM1. Western blotting showed that the highly expressed ECM1 protein was mainly ECM1a subtype, with a relative molecular mass of 68 000. Conclusion: ECM1, mainly ECM1a subtype, is overexpressed in breast cancer tissues and cell lines of high malignancy, which is associated with the metastasis of breast cancer.

国家自然科学基金资助项目(No. 30080027)；上海市基础研究重大项目基金资助（No.02JC14005）；上海市松江区科学技术攻关基金资助项目（No.06KG27）