摘 要 目的:研究人微小RNA10a（microRNA10a，miR10a）对胃癌细胞系BGC823迁移和侵袭能力的影响。方法：利用Transwell小室对胃癌细胞系BGC823进行侵袭筛选，获得高侵袭能力的BGC823P3亚系；通过miRNA芯片差异分析发现miR10a在高侵袭能力BGC823P3细胞的表达显著高于BGC823细胞。通过化学方法合成成熟型的人miR10a，以脂质体包裹合成的miR10a（25、50、100、150 nmol/L）转染BGC823细胞，并设空白转染、无关序列转染对照组; Realtime PCR分别检测以上各组细胞miR10a的表达。采用细胞计数试剂盒8（Cell Counting Kit8，CCK8）检测miR10a对细胞增殖的影响，流式分析检测miR10a对细胞凋亡的影响，Transwell小室检测miR10a对细胞的迁移和侵袭能力的影响。结果：化学合成的成熟型miR－10a转染后，BGC823细胞miR10a表达的提高以100 nmol/L miR10a转染组最佳，较无关序列转染组提高了2.06倍。 miR10a（１００ nmol/L）的转染对胃癌细胞系BGC823的增殖和凋亡无明显影响，但对BGC823的迁移和侵袭能力有明显的促进作用，促进率分别为（88.34± 0.61）% 和（56.02±3.13）%。结论：转染成熟型人miR－10a能使胃癌细胞系BGC823中miR10a的表达提高，并能显著促进胃癌细胞系BGC823的迁移和侵袭。

Abstract Objective: To investigate the effect of human microRNA10a(miR10a) on the migration and invasion of gastric cancer cell line BGC823. Methods: The Transwell system was used to select highly invasive subcell lines from gastric cancer cell line BGC823. Using miRNA microchip, we compared the miRNA expression in paired cell lines with high and low invasive potentials. MiR10a was relatively overexpressed in the highly invasive cell lines when compared with its counterpart. The mature type human miR10a was synthesized chemically. The synthesized miR10a（25, 50, 100, and 150 nmol/L）was transfected into BGC823 cells via lipofectamin 2000. Cells were also transfected with empty vectors and unrelated fragment to serve as controls. The expression of mature type miR10a was detected by realtime PCR. Cell counting kit8 was used to study the effect of miR10a on the proliferation of BGC823 cells. Flow cytometry was performed to detect the effect of miR10a on the apoptosis of BGC823 cells. The migration and invasion of BGC823 cells were investigated by Transwell assay. Results: Realtime PCR showed that cells transfected with mature type miR10a had significantly higher expression of miR10a, with the optimal concentration of miR10a being 100 nmol/L; the associated miR10a expression was 2.06 folds that of unrelated group. miR10a (100 nmol/L) had no obvious influence on the proliferation and apoptosis of BGC823 cells; however, it promoted the migration and invasion of BGC823 cells, with the promoting rates being (88.34±0.61)% and (56.02±3.13)%, respectively. Conclusion: Synthesized mature type human miR10a can effectively enhance miR10a expression and promote the migration and invasion of the BGC823 cells.

国家自然科学基金资助项目（No.30570818）；国家重点基础研究发展计划（973）资助项目（No.2009CB521804）