摘 要 目的: 肝细胞癌中高表达磷脂酰肌醇蛋白聚糖3基因(glypican3,GPC3)，而在非肿瘤肝组织、肝细胞腺瘤、胆管癌、肝内胆管细胞癌、胆囊癌等细胞中低表达甚至不表达；本研究利用携带GPC3重组真核表达载体，探讨GPC3基因对SKHep1肝癌细胞增殖、黏附和侵袭能力的影响。方法：将pEGFP-N2-GPC3通过脂质体方法转染人肝癌细胞SKHep1。RT-PCR检测GPC3SKHep1细胞中GPC3 mRNA的表达；MTT法检测SK-Hep-1细胞的增殖并计算黏附率；Transwell小室实验检测SK-Hep-1肝癌细胞的迁移能力和侵袭能力。结果：pEGFPN2GPC3成功转染SKHep1细胞，转染后GPC3-Hep-1细胞明显表达GPC3mRNA。GPC3转染能显著抑制肝癌细胞SK-Hep-1的增殖（P<0.01）；GPC3转染细胞的黏附能力较对照细胞显著下降[（10.21±0.62）% vs (15.51±095)%,P<0.01];GPC3转染细胞的迁徙和侵袭能力较对照细胞明显增强[（131.7±7.44）vs（69.6±5.25），P<0.01;(220±12.8) vs （130±8.2），P< 0.01]。结论：GPC3基因显著抑制肝癌细胞的增殖和黏附能力，但显著增强后者的迁移和侵袭能力。

Abstract Objective: To study the influence of GPC3 gene on the proliferation, adhesion and invasion of hepatoma cell line SKHep1. Methods: SKHep1 cells were transfected with pEGFPN2GPC3 using Lipofectamine2000. RTPCR was used to examine the GPC3mRNA expression in GPC3SKHep1 cells. MTT assay was used to examine the proliferation and calculate the adhesion rate of SKHep1 cells. Transwell system was used to assess the migration and invasion of the cells. Results: SKHep1 cells were successfully transfected with pEGFPN2GPC palsmid. GPC3 mRNA was detected in SKHep1 cells. Transfection with CPC3 significantly suppressed the growth of SKHep1 cells（P<0.01）. The adhesion ability of GPC3transfected cells was significantly decreased compared with control group（ [10.21±0.62]% vs [15.51±0.95]%, P<0.01]. Transfection with GPC3 significantly enhanced migration and invasion capacity（[131.7±7.44] vs [69.6±5.25]，P<0.01; [220±12.8] vs [130±8.2]，P<0.01]. Conclusion: Transfection with GPC3 gene can greatly inhibit the proliferation and adhesion of hepatoma SKHep1 cells, but can enhance their migration and invasiveness. The present study provides an experimental basis for studying the invasion mechanism of liver cancer.

福建省青年科技人才创新项目（No.2005J074）