目的: 筛选能提高鼻咽癌CNE2细胞对β射线放射敏感性的CpG ODN序列，观察筛选获得的CpG ODN序列放射增敏的作用特点，为寻找新的鼻咽癌放疗增敏剂提供实验依据方法：应用MTT实验筛选21种CpG ODN序列中对人鼻咽癌CNE2细胞放射增敏作用最强的序列，以MTT实验、集落形成实验、划痕实验和流式细胞术检测该最强作用序列与β射线联合作用对CNE2细胞的增殖、集落形成、细胞迁移、细胞周期与凋亡的影响。结果：筛选实验显示CpG ODN107对 CNE2细胞具有最高的增敏比（1.59±0.06），CpG ODN107和β射线联合作用能以剂量依赖方式显著抑制CNE2细胞的增殖，抑制效应显著高于单纯照射（P<0.05, P<0.01）；联合作用显著抑制CNE2细胞集落形成和迁移能力，显著诱导细胞周期阻滞于G0/G1期，显著增加细胞凋亡（P<0.01），上述作用效应均明显强于单纯β射线照射（P<0.05或P<0.01）。结论： CpG ODN 107可显著增强人鼻咽癌CNE2细胞对β射线的照射敏感性，其放射增敏作用可能与诱导细胞周期阻滞和凋亡有关。

Objective:To screen for specific CpG ODN sequence which can enhance the radiosensitivity of nasopharyngeal carcinoma cell line CNE2 cells to βray and to observe the sensitivityenhancing characters of the screened CpG ODN sequence, so as to provide evidence for searching novel sensitizer of nasopharyngeal carcinoma to radiation. Methods: MTT assay was used to select the strongest sequence to sensitize CNE2 cells to βray irradiation from 21 CpG ODN sequences. The effect of the selected sequence combined with βray irradiation were used to treat CNE2 cells and the proliferation, colony forming, cell migration, cell cycle and apoptosis were all observed. Results: Screen assay found CpG ODN107 had the highest SER (1.59±0.06) for CNE2 cells. CpG ODN107 combined with βray irradiation inhibited CNE2 cell proliferation in a dose-dependent manner, and the inhibitory effect was significantly higher than that of radiation alone (P<0.05, P<0.01). CpG ODN107 combined with βray irradiation significantly inhibited the formation and migration of CNE2 cell colony, and induced G0/G1 arrest and significantly increased cell apoptosis compared with that of radiation alone (P<0.05 or 0.01). Conclusion：Our results suggest that CpG ODN107 can greatly enhance the radiosensitivities of human nasopharyngeal carcinoma cell line CNE2 to βray radiation, which might be related to the induction of cell cycle arrest and apoptosis.

国家自然科学基金资助项目（No.30271512）。