目的: 探讨青春型双歧杆菌对兔VX2瘤组织HIF1α、VEGF表达的影响。方法: 建立兔荷VX2瘤模型20只，随机分成2组，每组10只。对照组经耳缘静脉注射生理盐水，实验组经耳缘静脉注射青春型双歧杆菌。处死动物，取出脏器和肿瘤组织粉碎后进行厌氧培养和革兰染色，观察双歧杆菌对肿瘤的靶向性；另取部分肿瘤组织HE染色观察肿瘤组织的形态学变化；应用免疫组织化学方法分别检测肿瘤组织中HIF1α、VEGF的表达。结果: 青春型双歧杆菌能够靶向定植于肿瘤组织中；经双歧杆菌治疗后实验兔肿瘤组织坏死面积明显增加。免疫组化显示实验兔VX2瘤组织中HIF1α、VEGF阳性表达细胞密度显著低于对照组（P<0.05），且两组中HIF1α与VEGF的表达呈显著正相关（r=0.86）。结论: 青春型双歧杆菌能够在兔VX2瘤组织中靶向定植，且能下调兔VX2瘤中HIF1α和VEGF的表达

Objective:To investigate the influence of Bifidobacterium adolescence on the expression of HIF1α and VEGF in rabbit VX2 tumor. Methods: Rabbit VX2 tumor models were established and were divided into two groups (n=10). Animals in the experimental group were injected with Bifidobacterium adolescence and those in the control group received normal saline via ear vein. Animals were sacrificed and the liver, kidney, spleen, heart, lung and tumor tissues were removed, cultured and Gram stained. The changes of tumor tissue were observed by HE staining; immunohistochemistry was used to detect the expression of HIF1α and VEGF in tumor tissues. Results:Bifidobacterium adolescence could target the tumor tissue. HE staining showed the necrotic area was obviously enlarged after treatment with Bifidobacterium. Immunohistochemistry demonstrated that the positive rates of HIF1α and VEGF in the experimental group were significantly lower than those of the control group(P<0.05), and the expression of the HIF1α and VEGF in two groups was positively correlated (r=0.86). Conclusion:Bifidobacterium adolescence can specifically target rabbit VX2 tumors tissues, and decrease the expression of HIF1α and VEGF.

山东省科技厅博士基金项目（No. 2006BS03035）。