目的: 探讨VEGFR3对人结肠癌细胞黏附力和侵袭性的影响。方法:构建携靶向 VEGFR3基因siRNA(small interfering RNA)表达载体，转染人结肠癌LoVo细胞， 半定量RTPCR和Western blotting检测转染前后LoVo细胞VEGFR3mRNA和蛋白表达的变化，基质黏附实验检测细胞转染后的黏附能力，细胞侵袭实验检测转染后肿瘤细胞侵袭性的改变。结果:携靶向 VEGFR3 基因siRNA的表达载体成功构建，RTPCR检测转染siRNA后LoVo细胞 VEGFR3mRNA表达水平降低；Western blotting检测转染siRNA后72 h LoVo细胞VEGFR3蛋白表达下降，其表达相对值由(1.26±0.19)降至(0.39±012)(P<0.05)。转染siRNA 72 h后LoVo细胞的黏附能力显著下降\[（0.626±0.047）vs (0.407±0.029), P＜0.05\]；LoVo细胞穿膜细胞数(6.38±3.25)明显低于空白对照组(24.82±3.44)、非特异性对照组(23.58±3.73) (P＜0.05)。结论:siRNA能够在LoVo细胞中引发RNA干扰效应，下调VEGFR3基因的表达，进而抑制LoVo细胞的黏附能力和侵袭性。

Objective:To construct a small interfering RNA (siRNA) expression vector (psiRNAVEGFR3) targeting vascular endothelial growth factor receptor 3 (VEGFR3) and to investigate the effects of VEGFR3 siRNA on the adherence and invasion of human colon cancer cells. Methods: A siRNA expression vector (psiRNAVEGFR3) targeting VEGFR3were constructed and was used to transfect LoVo cells via lipofectamine 2000. The mRNA and protein expression of VEGFR3were examined after transfection by reverse transcriptase polymerase chain reaction (RTPCR) and Western blotting, respectively. The tumor adhesion ability was detected by cellmatrix adhesion experiment and the invasion ability of tumor cells was evaluated by millicell chamber model. Results: The VEGFR3 siRNA expression vector was successfully constructed. The expression of VEGFR3mRNA and protein was inhibited after psiRNAVEGFR3 transfection. Seventytwo hours after psiRNAVEGFR3 transfection, Western blotting assay showed that the expression of VEGFR3 protein was decreased from (1.26±0.19) to (0.39±0.12)(P＜0.05), the adhesion ability of LoVo cells was also significantly decreased compared with the untransfected group and negative control group (0.407±0029 vs 0.626±0.047,0.621±0.068, P<0.01). The invasion assay demonstrated that the number of LoVo cells penetrating the membrane in the transfection group was significantly lower than those in the untransfected and negative control group (6.38±3.25 vs 24.82±3.44, 23.58±3.73, P<0.05). Conclusion: The siRNA of VEGFR3gene can effectively inhibit the mRNA and protein expression of VEGFR3 in LoVo cells, therefore restraining the adhesion and invasion ability of LoVo cells.