目的: 探讨NogoB基因在人脑胶质瘤组织中的表达及其与胶质瘤病理分级的相关性。方法：收集上海长征医院神经外科2005-2008年手术获得的经病理证实的人脑胶质瘤组织标本36份，另取其他手术切除的正常脑组织标本10份。以免疫组化技术、Realtime PCR和Western blotting等方法检测这些标本中NogoB在基因和蛋白质层面的表达水平，结合病理资料分析NogoB的表达和病理分级的相关性。结果：Realtime PCR检测发现，恶性程度高的胶质瘤组织中NogoB基因表达明显下调，Ⅲ级的胶质瘤组织中NogoB基因表达水平与正常脑组织相比下降了90%左右(P<0.01)，NogoB基因表达水平与肿瘤的恶性度成相关。Western blotting检测显示，正常脑组织中NogoB蛋白表达相对恒定；在高恶性级别的胶质瘤中NogoB的蛋白表达显著下调，其下降程度与肿瘤的恶性程度呈负相关。免疫组织化学检测显示，NogoB主要在人正常脑组织中弥漫性阳性表达（100%），在人胶质瘤组织中表达显著降低；36例胶质瘤组织中NogoB染色阳性程度随着肿瘤恶性程度增加而显著降低（在ⅠⅡ、Ⅲ、Ⅳ级中阳性表达分别为88.2%，42.9%，25.0%），各组间比较差异有统计学意义（P<0.01）。结论：在人脑胶质瘤中NogoB在mRNA和蛋白质水平上表达均出现下调，其下调程度与病理分级呈负相关。

Objective: To explore the expression of NogoB in brain gliomas and its relationship with the pathological grades of gliomas. Methods: The expression of NogoB was investigated in 10 normal brain tissues and 36 brain glioma specimens, which were confirmed after surgery during 2005-2008 in Changzheng Hospital, using immunohistochemical method, realtime PCR and Western blotting assay．The relationship between NogoB expression and pathological grades were analyzed. Results:Tissue NogoB immunohistochemical staining in gliomas specimens suggested the involvement of NogoB in the proliferation of gliomas. The realtime PCR demonstrated that NogoB mRNA expression level in gliomas was obviously decreased in gliomas of high pathological grades, and the expression was stable in normal brain tissues. The expression of NogoB was negatively correlated with the malignancy of glioma. The expression in gliomas of grade Ⅲ decreased by 90% compared with the normal brain tissues (P<0.01). Western blotting showed that NogoB protein expression was stable in normal brain tissues and obviously decreased in glioma tissues of high grade; the decrease was negatively correlated with the malignancy of gliomas. Immunohistochemical analysis showed NogoB expression was scattered in the normal brain tissues (100%) and greatly decreased in human gliomas. The expression of NogoB was decreased with the increase of the malignancy in our 36 specimens, being 88.2%，42.9%，and 25.0% in ⅠⅡ,Ⅲ, and Ⅳ grades, respectively (P<0.01)．Conclusion: NogoB is decreases in human glioma at both protein and mRNA level. NogoB participats in the development and progression of bliomas, and its downregulation is closely related to the malignancy of tumors.

国家高技术研究发展(863)计划资助项目（No.2007AA02Z483）；第二军医大学青年启动基金资助项目（No.07QN29）.