目的: 探讨潜活膜蛋白1（latent membrane protein 1, LMP1）、NET1、血管内皮生长因子（vascular endothelial growth factor, VEGF）在非角化性鼻咽癌（nonkeratin nasopharyngeal carcinoma, NKNPC）中的表达及其临床意义。方法: 取南通大学附属医院1999年5月－2003年5月收治的病理诊断明确的60例NKNPC活检标本，采用免疫组织化学技术（Envison二步法）检测LMP1、NET1、VEGF的表达，并分析其与临床病理参数和预后的相关性。另取10例鼻咽慢性黏膜炎症组织作对照。结果: （1）NKNPC中LMP1、NET1、VEGF的表达显著高于鼻咽慢性黏膜炎症组织 (P<0.05)；（2） NKNPC中，LMP1表达强度与有无远处转移密切相关(P<0.05)，与患者性别、年龄、T分期、N分期无显著相关性(P＞0.05)；NET1、VEGF表达强度与淋巴结转移，远处转移密切相关(P<0.05 )，与患者性别、年龄、T分期均无显著相关性(P＞0.05)。（3）NKNPC中LMP1、NET1、VEGF的表达呈显著相关性 (P<0.05)。（6）单因素KaplanMeier生存曲线分析显示，LMP1、VEGF表达与NKNPC的生存率之间的相关性有显著统计学意义(P<0.05)。结论: LMP1、NET1、VEGF的联合检测有助于预测NPC转移与预后，LMP1和VEGF可作为评估NKNPC预后的指标之一。

Objective:To investigate the expression of latent membrane protein1 (LMP1) , NET1, and vascular endothelial growth factor (VEGF) in nonkeratin nasopharyngeal carcinoma (NKNPC) and its clinical significance. Methods:Sixty biopsy specimens from pathologicallyconfirmed NKNPC patients, who were treated in the Affilitated Hospital of Nantong University from May 1999 to May 2003, were included in the present study. Using immunohistochemical techniques (Envison twostep), we examined the expression of LMP1, NET1 and VEGF protein in the specimens. The relationship between their expression and clnicopathological parameters and the prognosis was anayzed. Ten specimens of chronic nasopharyngitis served as control. Results:(1) The positive rates of LMP1, NET1 and VEGF in NKNPC were significantly higher than those in the chronic nasopharyngitis (P<0．05). (2) The LMP1 expression in NKNPC specimens were positively correlated with distant metastasis of NKNPC (P<0．05)，and not correlated with the sex，age，T stages and N stages (P＞0．05)． (3) NET1 and VEGF expression in NKNPC specimens were positively correlated with lymph node metastasis and distant metastasis (P<0.05), and not correlated with the sex，age，and T stages． (4) The expression of LMP1, NET1, and VEGF was correlated with each other (P<0.05)．(5) Kaplan-Meier survival curve analysis showed that the expression of LMP1, VEGF was significantly correlated with the prognosis of NKNPC patients (P<005)．Conclusion: Combined examination of LMP1, NET1, and VEGF can help to predict NPC metastasis and prognosis. LMP1 and VEGF might be used as an indicator for prognosis of NKNPC．