目的:研究IL27对肿瘤血管生成的抑制作用及其机制。方法： IL27基因稳定转染的人食管癌细胞（Eca109/IL27）接种于裸鼠，建立荷瘤裸鼠模型，观察肿瘤生长情况和裸鼠生存期。用ELISA法检测脾细胞IFNγ的分泌水平；免疫组化法检测瘤组织中VEGF和CD34的表达，并通过CD34的水平计算微血管密度；用RTPCR法检测肿瘤组织趋化因子IP10、MIG mRNA的表达水平。结果：接种Eca109/IL27细胞荷瘤小鼠的生存期较接种野生型Eca109细胞（未转染质粒）和Eca109/LXSN细胞（空载体质粒转染）小鼠的生存期明显延长（P<0.05）。接种Eca109/IL27细胞的裸鼠瘤组织中VEGF和CD34的表达水平显著性低于接种Eca109细胞和Eca109/LXSN细胞，微血管密度显著降低（均P<0.01）。Eca109/IL27组小鼠脾细胞产生较高水平的IFNγ（P<0.05），趋化因子IP10和MIG mRNA的表达水平也显著性高于接种Eca109细胞组和Eca109/LXSN细胞组（P<0.05）。结论： IL27在裸鼠体内通过上调IP10和MIG表达抑制肿瘤血管生成，从而发挥抗肿瘤作用。

Objective: To study the inhibitory effect of IL27 against human tumor angiogenesis and the related mechanisms. Methods: Human esophageal carcinoma cells (Eca109/IL27) stably transfected with IL27 gene were injected into nude mice to establish tumorbearing mouse model. The survival time and tumor growth were observed. IFNγ level secreted by splenocytes was measured by ELISA. Expression of VEGF and CD34 was detected by immunohistochemistry method and MVD was calculated according to CD34 level. RTPCR was used to detect the expression of IP10 and MIG mRNA in the tumor tissues. Results: The survival time of mice injected with Eca109/IL27 cells was significantly longer than those of mice injected with wide type Eca109 or Eca109/LXSN (blank vector) cells (P<0.05). Expression of VEGF and CD34 in Eca109/IL27inoculated mice was lower than those in Eca109 or Eca109/LXSN groups. Production of IFNγ by splenocytes in mice injected with Eca109/IL27 cells was higher than those with Eca109 and Eca109/LXSN (P<0.05). MIG and IP10 mRNA expression was also higher than those in Eca109 or Eca109/LXSN injected mice (P<005). Conclusion: IL27 can inhibit tumor angiogenesis in nude mice through upregulating the expression of MIG and IP10, and thus exerts antitumor effect

科技部重大科技攻关课题 （No. 2006BAI02A07）