目的:探讨苯丁酸钠(sodium phenylbutyrate，NaBP)对甲状腺滤泡癌细胞CGTHW1的侵袭能力及基质金属蛋白酶9(matrix metalloproteinase9, MMP9)和金属蛋白酶组织抑制剂1(tissue inhibitor of metalloproteinase,TIMP1)表达的影响 。方法：培养CGTHW1细胞，通过Transwell侵袭实验观察苯丁酸钠对CGTHW1细胞侵袭能力的影响，采用免疫细胞化学SP法及RTPCR观察苯丁酸钠对CGTHW1细胞中MMP9和TIMP1蛋白及mRNA表达的影响 。结果： 4 mmol/L苯丁酸钠作72 h， CGTHW1 细胞的侵袭细胞数显著减少\[（29.8±1.77）vs（11.00±2.59），(P<0．01)\]。免疫细胞化学和RTPCR 检测结果显示，4 、6 mmol/L 苯丁酸钠显著抑制CGTHW1细胞中MMP9、TIMP1蛋白及mRNA的表达 (P<0．05)。结论：苯丁酸钠可通过下调MMP9和TIMP1的表达进而降低甲状腺滤泡癌CGTHW1细胞的侵袭能力。

Objective: To investigate the effects of sodium phenylbutyrate (NaPB) on the matrix metalloproteinase9(MMP9) and tissue inhibitor of metalloproteinase1(TIMP1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW1. Methods: CGTHW1 cells were treated with different concentrations of NaPB, then the invasive ability of CGTHW1 cells was assessed using Transwell assay. The expression of MMP9 and TIMP1 was examined by immunocytochemistry staining and RTPCR in CGTHW1 cells. Results: After treatment with NaPB (4 mmol/L) for 72 h, CGTHW1 cells passing the Transwell were significantly reduced\[(29.8±1.77) vs (11.00±2.59)，P<005\]. Immunocytochemistry staining and RTPCR demonstrated that protein and mRNA expression of MMP9 and TIMP1 were significantly downregulated in CGTHW1 cells after NaPB treatment (4, 6 mmol/L) (all P<005).Conclusion: NaPB can inhibit CGTHW1 cell invasion by downregulating MMP9 and TIMP1 expression.

锦州市科学技术计划项目（No.08B1E28)