目的:探讨以骨髓间充质干细胞（mesenchymal stem cells，MSCs） 为基因治疗载体表达外源性IL12对胶质瘤C6细胞增殖的影响。方法：分离培养大鼠MSCs, 腺病毒介导IL12基因转染大鼠MSCs(AdIL12MSCs),RTPCR 及Western Blotting检测AdIL12MSCs中IL12基因mRNA及蛋白表达。MTT法检测AdIL12MSCs分泌的外源性IL12对C6胶质瘤细胞增殖活性 的影响，光镜下观察外源性IL12对C6细胞形态的影响。结果：腺病毒介导IL12基因成功转染MSCs形成AdIL12MSC,其IL12基因在mRNA及蛋白水平均有明显表达。AdIL12MSC分泌的外源性IL12显著抑制胶质瘤C6细胞的增殖（P<0.05）。结论：转染IL12的MSCs(AdIL12MSC)能够在mRNA及蛋白水平表达外源性IL12基因,显著抑制胶质瘤C6细胞的增殖。

Objective: To study the influence of mesenchymal stem cells (MSC) infected by AdIL12 on the proliferation of C6 glioma cells. Methods: The MSCs were cultured from rat bone marrow and verified by immunohistochemistry and flow cytometry. Recombinant adenovirus vectors harboring IL12 (AdEasyIL12) were infected into MSCs to construct AdIL12MSC containing exogenous IL12. RTPCR and Western Blotting were used to detect IL12 mRNA and protein expression in AdIL12MSC. MTT method was used to detect the effect of AdIL12MSC supernatant on the proliferation of C6 glioma cells. Results: Exogenous IL12 gene was effectively transfected into MSCs by recombinant adenovirus vectors. IL12 was expressed in MSCs at both mRNA and protein levels as detected by RTPCR and Western Blotting. The supernatant of AdIL12MSC significantly inhibited the proliferation of C6 glioma cells compared with MSC supernatant（P<0.05）. Conclusion: MSCs tansfected with IL2 gene (AdIL12MSC) can express IL12 at mRNA and protein levels and inhibit the proliferation of C6 glioma cells.

锦州市科学技术计划项目（No.08B1E28)