目的: 探讨Apoptin对人肝癌细胞株HepG2及C57BL/6小鼠H22移植瘤的抑制作用。方法：应用脂质体转染法将重组质粒pVAX1Apoptin转染HepG2细胞，应用Western blotting检测Apoptin蛋白在转染后HepG2细胞中的表达，应用MTT检测Apoptin对HepG2细胞生长的抑制作用，通过AO/EB染色法检测pVAX1Apoptin致肿瘤细胞凋亡作用。建立C57BL/6小鼠H22荷瘤模型，瘤内注射pVAX1Apoptin，观察pVAX1Apoptin对体内肿瘤的抑制作用。结果： Apoptin基因可在HepG2细胞中有效表达，pVAX1Apoptin能够诱导HepG2细胞凋亡，并显著地抑制其生长，48 h抑制率为69.28%。pVAX1Apoptin瘤内注射能够有效抑制移植肿瘤的生长，抑制率为46.71%；治疗后39 d小鼠生存率为40%，显著高于对照组(P<005)。结论： Apoptin转染可抑制HepG2细胞的生长，重组质粒pVAX1Apoptin瘤内注射对移植肿瘤有显著的抑制作用。

Objective:To investigate the antitumor effects of Apoptin against human hepatocellular carcinoma HepG2 cells and implanted H22 tumors in C57BL/6 mice. Methods: C57BL/6 mice were used to establish H22bearing models. pVAX1Apoptin was intratumorally injected and the inhibition of tumor growth was observed. Recombinant plasmid pVAX1Apoptin was transfected into HepG2 cells by Lipofectamine. The expression of Apoptin protein in HepG2 cells was examined by Western blotting. Antitumor effect of pVAX1Apoptin on HepG2 cells was measured by MTT assay, and the apoptosis of HepG2 cells after transfected with pVAX1Apoptin was observed by AO/EB staining.Results:Apoptin gene was effectively expressed in HepG2 cells after transfection with pVAX1Apoptin. pVAX1Apoptin induced apoptosis of HepG2 cells and inhibited the growth of HepG2 cells, with the suppression rate being 69.28% at 48 h after transfection. Intratumoral injection of pVAX1Apoptin significantly inhibited tumor growth, with tumor inhibition rate being 46.71% and mice survival rate being 40% at 39 d after injection. Conclusion: pVAX1Apoptin can inhibit the proliferation of HepG2 cells, and intratumoral injection of pVAX1Apoptin can greatly inhibit tumor growth in vivo.

国家高技术研究发展计划(863)资助项目(No. 2007AA021004)