目的:观察人结直肠癌组织和细胞株中ATPase F1α的表达，并探讨其意义。方法: 应用免疫组化EnVision法测定收集自长海医院2007年8月至12月的44例结直肠癌组织及癌旁组织手术切除标本中ATPase F1α的表达；半定量RTPCR方法检测8例结直肠癌及癌旁组织中ATPase F1α mRNA的表达水平。采用免疫荧光法检测ATPase F1α在人结肠癌LoVo细胞株胞膜的表达；采用CCK8活细胞计数法检测抗ATPase F1α抗体对LoVo细胞增殖的抑制作用。结果:44例结直肠癌组织中ATPase F1α的表达显著高于癌旁组织(P<0.01)；44例结直肠癌组织中ATPase F1α的中高度表达有35例（795%），癌旁配对组织的中高度表达为15例（34.1%）。8例结直肠癌组织中ATPase F1α mRNA的表达明显高于癌旁组织（P<001）。ATPase F1α在结直肠癌组织中的阳性表达率高于患者术前血浆CEA阳性率（P<0.01），而与患者年龄、性别、肿瘤的临床分期、淋巴结转移、远处转移、肿瘤的部位、分化程度等均无关（P>0.05）。结肠癌LoVo细胞株胞膜上可见颗粒状分布的ATPase F1α，ATPase F1α抗体对LoVo细胞的增殖活性有明显的抑制作用（P<0.01）。结论: 人结直肠癌组织和细胞株均高表达ATPase F1α，其作为肿瘤抗原，有可能成为结直肠癌靶向治疗的新靶点。

Objective: To observe the expression of ATPase F1α in colorectal cancer (CRC) tissues and in LoVo cells, and to discuss its clinical significance. Methods: Expression of ATPase F1α protein in 44 CRC specimens and their adjacent normal tissues (August 2007 to December 2007, Changhai Hospital) and ATPase F1α mRNA in 8 colorectal cancer tissues and their adjacent normal tissues were examined by immunohistochemistry EnVision assay and RTPCR, respectively. Expression of ATPase F1α on the cell surface of LoVo cells was observed by immunofluorescence. The inhibitory effect of antiATPase F1α antibody on the proliferation of LoVo cells was evaluated by CCK8 assay. Results: Expression of ATPase F1α in the 44 CRCs were significantly higher than those in the adjacent normal tissues as detected by immunohistochemistry (P<0.01). Thirtyfive samples (79.5%) in 44 CRCs and 15 samples (34.1%) in their adjacent normal tissues showed moderate to high expression of ATPase F1α. Expression of ATPase F1α mRNA in 8 CRCs was significantly higher than those in their adjacent normal tissues (P<0.01). Positive rate of ATPase F1α mRNA in CRCs was significantly higher than that of CEA in the same patients before surgery (P<0.01). There was no correlation of ATPase F1α expression with the age, gender, tumor stage, lymphoid metastasis, remote metastasis, tumor location and tumor differentiation of colorectal cancer (P>0.05). Expression of ATPase F1α was observed on the cell surface of LoVo cells, and antiATPase F1α antibody significantly inhibited the proliferation of LoVo cells (P<0.01). Conclusion: ATPase F1α is highly expressed in colorectal cancer tissues and cell line. As a tumor association antigen, ATPase F1α may serve as a new target in colorectal cancer immunotherapy.

全军医学科研“十一五计划”资助项目(No.01MA159)