目的: 用自制的改良细胞冻存液冻存树突状细胞（dendritic cells，DCs），观察冻存复苏后DCs的细胞存活率及体外诱导CIK(cytokine induced killer cell)对肿瘤细胞的杀伤作用。方法：从外周血分离、培养获得DCs，分别用3种冻存液冻存:（1）含10%二甲基亚砜(DMSO)、20%牛血清的RPMI 1640培养液；（2）日本ZENOAQ公司的Cellbanker细胞冻存液；（3）自制细胞冻存液（含DMSO、羟乙基淀粉及细胞稳定剂）。每组DCs分6管,于-80 ℃和-196 ℃各冻存3管,分别于冻存后第30、60和180 d复苏，用锥虫蓝染色法测定细胞存活率，用MTT法检测冻存复苏后DCs活化的CIK对K562细胞的杀伤活性。结果：每组3种冻存液冻存的DCs随着冻存时间的延长，冻存复苏DCs的存活率和活性均有轻度下降，但经统计学分析3种冻存液的冻存效果无明显差别。结论：自制的改良细胞冻存液能够替代传统冻存液和进口冻存液用于DCs的冻存，有良好的推广前景

Objective:To observe the survival rate and activity of dendritic cells (DCs) to induce the activation of cytokine induced killer cell (CIK) after DCs being preserved in modified cell freezing medium (CFM). Methods:PBMCderived DCs were preserved in three different CFMs at -80 ℃ and -196 ℃, respectively. CFM I was RPMI 1640 containing 10% DMSO, 20% FCS; CFM Ⅱ was Cellbanker (ZENOAQ company, Japan); CFM Ⅲ was modified CFM containing DMSO, hydroxyethylamyle and cell stabilizer. Survival rate of DCs and antitumor activity of DCs activatied CIK (DCCIK) were measured by Trypan blue dye exclusion method and MTT after DCs being preserved in three different CFMs for 30, 60 and 90 d at -80 ℃ or -196 ℃, respectively. Results: Survival rate and activity of DCs preserved in three different CFMs were only slightly reduced as storage period was increased, and there were no significant differences among three CFMs. Conclusion:Modified CFM can substitute traditional and imported Cellbanker CFM in DCs freezing with bright future.