目的：研究淫羊藿苷（icariin，ICA）体外对脐血单个核细胞来源树突状细胞的分化、成熟及免疫活性的影响。方法：无菌条件下采集脐血，密度梯度离心法分离脐血单个核细胞，用GMCSF、IL4诱导树突状细胞（dendritic cells, DCs），第5天后将DCs分为3组（ICA组、TNFα组和阴性对照组）并作相应处理。倒置显微镜和透射电镜下观察DCs形态，流式细胞术检测DCs表面分子CD1a、CD80、CD83和CD86的表达情况，ELISA法检测DCs培养上清中IL12和IFNγ的水平，MTT法检测DCs刺激T细胞增殖的能力。结果：ICA刺激后，脐血单个核细胞来源DCs呈现典型的成熟DCs形态学特征。ICA组DCs表面CD1a、CD80、CD83和CD86表达水平较对照组均明显上调（P<0.05），且CD86的表达水平显著高于TNFα组（P<0.05）。ICA组DCs上清中IL12、IFNγ的水平及诱导T细胞增殖的能力较对照组DCs明显增高（P<0.05），但与TNFα组DCs无显著差异。结论：ICA可刺激脐血单个核细胞来源DCs的分化和成熟，增强DCs的免疫学活性。

Objective:To investigate the in vitro effect of icariin (ICA) on the differentiation, maturation and immune activity of DCs derived from cord blood mononuclear cells.Methods: The mononuclear cells were isolated by density gradient centrifugation from cord blood under axenic condition, and were induced by GMCSF and IL4 to differentiate into DCs. The DCs were divided into 3 groups on the fifth day: an ICA group, a TNFα group and a control group. The morphology of DCs was observed under inverted microscope and transmission election microscope. Expression of CD83, CD80 and CD86 on DCs was examined by flow cytometry. IL12 and IFNγ levels in the culture supernatant of DCs were detected by ELISA. The proliferation of T cells stimulated by DCs was determined by MTT assay.Results: Cord blood mononuclear cells derivedDCs had a typical morphological characteristic of mature DCs after stimulation with ICA. Expression of CD1a, CD83, CD80 and CD86 on DCs in ICA group was upregulated compared with that in control group (P<0.05); moreover, CD86 expression on DCs in ICA group was significantly higher than that in TNFα group (P<0.05). IL12 and IFNγ production in ICA group and proliferation of T cells induced by DCs in the ICA group were markedly higher than those in the control group (P<0.05), and were similar to those in the TNFα group.Conclusion: ICA can induce differentiation and maturation of DCs derived from cord blood mononuclear cells, and enhances the immune activity of DCs.

河北省自然科学基金资助项目（No. C2008000952）