目的:研究转录因子激活蛋白2α基因（transcription factor activator protein2α,AP2α）对大肠癌SW620细胞侵袭生长的影响，并探讨其对雌激素受体β基因（estrogen receptorβ, ERβ）表达的影响及其可能的分子机制。方法：利用脂质体介导重组质粒pcDNA3.1(+)AP2α与空质粒pcDNA3.1(+)转染入SW620大肠癌细胞，采用基质胶黏附实验与Transwell实验检测细胞的体外黏附、侵袭生长与迁移能力,采用实时定量PCR、Western blotting、免疫荧光细胞化学检测细胞中AP2α和ERβ在基因和蛋白水平的表达，以电泳迁移率分析（electrophoretic mobility shift assay, EMSA）方法检测转染AP2α基因后肿瘤细胞中AP2α的DNA结合活性及其与ERβ的结合能力。结果：AP2α转染SW620细胞后抑制了细胞的体外黏附、侵袭与迁移能力(均P<0.05)；同时细胞中ERβ基因的mRNA和蛋白表达水平显著升高(P<0.05)；EMSA结果显示，AP2α转染的SW620细胞中表达的AP2α蛋白与ERβ基因启动子区域发生特异性结合。结论：AP2α转染SW620细胞可显著抑制大肠癌SW620细胞体外黏附、侵袭与迁移能力，其分子机制很可能与AP2α直接作用于ERβ基因启动子区域从而影响ERβ基因的表达有关。

Objective:To study the effects of transcription factor activator protein2α (AP2α）on invasive growth and estrogen receptorβ (ERβ) expression in human colon cancer SW620 cells, and to probe into the involved molecular mechanism.Methods: Plasmid pcDNA3.1(＋) AP2α and pcDNA3.1(＋) were transfected into SW620 cells by liposomemediated transfection. The adhesion, invasion and migration abilities of SW620 cells were measured by metrical gel adhesion assay and modified Boyden chamber (Transwell assay). The gene and protein expression levels of AP2α and ERβ in SW620 cells were examined by Realtime PCR, Western blotting and immunofluorescence cytochemistry. The interaction between AP2α DNA and ERβ in SW620 cells was measured by electrophoretic mobility shift assay (EMSA) afterAP2αgene transfection.Results: Overexpression of AP2α markedly reduced the adhesion, invasion and migration abilities of SW620 cells (all P<0.05); meanwhile, the mRNA and protein levels of ERβ in SW620 cells were also significantly enhanced (P<0.05). EMSA results showed that AP2α specifically bound to promoter region of ERβ gene in SW620 cells after transfection of pcDNA3.1(+)AP2αplasmid. Conclusion: Overexpression of AP2α can inhibit the adhesion, invasion and migration abilities of SW620 cells, which is probably related to ERβ expression in SW620 cells directly induced by interaction between AP2α and promoter region of ERβ.

山西省科学技术发展计划项目（No.2006031087）