目的：探讨鸦胆子(Brucea javanica)油乳在体外对宫颈癌SiHa细胞的抑制作用及其作用机制。方法：以鸦胆子油乳（2.5、5.0、10、20、40、80 μg/ml）作用于SiHa细胞24、48、72 h,以MTT法检测鸦胆子油乳对SiHa细胞增殖的抑制作用；透射电镜观察细胞的超微结构；琼脂糖凝胶电泳观察SiHa细胞基因组DNA片段化改变；流式细胞术测定AnnexinV/PI双染色后SiHa细胞凋亡率。结果：MTT结果显示，鸦胆子油乳以剂量和时间依赖方式对SiHa细胞增殖有明显的抑制作用，其中80 μg/ml 药物作用72 h时的抑制率可达92.43%；20 μg/ml药物作用后，透射电镜下可观察到细胞出现凋亡小体，琼脂糖凝胶电泳显示细胞凋亡特征性的DNA片段化“梯状”条带；流式细胞仪检测显示，10、20、40 μg/ml药物组作用48 h后凋亡率分别为（8.02±241）%、（51.60±7.67）%、（77.22±5.80）%。结论：鸦胆子油乳能够抑制SiHa细胞增殖，其作用机制可能为诱导SiHa细胞发生凋亡。

Objective:To investigate the inhibitory effect of Brucea javanica oil emulsion on cervical cancer SiHa cells in vitro and the related mechanisms. Methods: SiHa cells were treated with Brucea javanica oil emulsion（2.5, 5.0, 10, 20, 40, and 80 μg/ml）for 24, 48, and 72 h. The inhibitory effect of Brucea javanica oil emulsion on the proliferation of SiHa cells was examined by MTT; the ultrastructure of SiHa cells was observed by transmission electron microscope (TEM); the DNA fragment of SiHa cells was detected by agarose gel electrophoresis; and the apoptosis of SiHa cells was examined by flow cytometry through Annexin V and PI staining. Results:MTT results showed that Brucea javanica oil emulsion significantly inhibited the proliferation of SiHa cells in a dose and timedependent manner. The inhibitory rate of SiHa cells reached 92.43% after treatment with 80 μg/ml Brucea javanica oil emulsion for 72 h. Apoptotic bodies and a characteristic apoptosis DNA ladder of SiHa cells were observed after treatment with 20 μg/ml Brucea javanica oil emulsion. Flow cytometry results demonstrated that the apoptosis rates of SiHa cells were (8.02±241)%, (51.60±7.67)%, and (77.22±580)% 48 h after treatment with 10, 20, and 40 μg/ml Brucea javanica oil emulsion for 48 h, respectively. Conclusion: Brucea javanica oil emulsion can inhibit the proliferation of SiHa cells, probably by inducing apoptosis of SiHa cells.

国家自然基金资助项目（No. 30670916）；辽宁省科学技术计划项目(No.200722500522)