目的：构建靶向存活素（survivin）的干扰RNA（siRNA）质粒，观察其对A549细胞增殖、凋亡以及顺铂敏感性的影响。方法：应用pSilencerU6质粒构建survivinsiRNA干扰质粒，RTPCR和Western blotting检测survivin mRNA和蛋白的表达，DAPI染色法检测细胞的凋亡，MTT法检测细胞的增殖。结果：成功构建了survivinsiRNA干扰质粒。SurvivinsiRNA质粒转染可明显下调A549细胞中survivin mRNA和蛋白的表达、抑制A549细胞的增殖、促进细胞的凋亡、增强A549细胞对顺铂的敏感性。结论：SurvivinsiRNA沉默survivin在肺癌细胞中的表达能够抑制肿瘤细胞的增殖、促进细胞凋亡，并能增强肿瘤细胞对化疗药物顺铂的敏感性，survivin 可作为肺癌治疗的潜在靶点。

Objective:To investigate the effect of survivinsiRNA plasmid on survivin expression in human lung cancer cell line A549, and to observe its effect on the apoptosis, proliferation, and chemosensitivity of A549 cells. :Methods: :pSilencersurvivinsiRNA (survivinsiRNA) plasmid was constructed using pSilencerU6 plasmid and was transfected into A549 cells. Expression of survivin mRNA and protein was examined by RTPCR and Western blotting analysis, respectively. Apoptosis and proliferation of A549 cells were examined by DAPI staining and MTT, respectively. :Results: :SurvivinsiRNA plasmid was successfully constructed, and it significantly inhibited survivin mRNA and protein expression in A549 cells. SurvivinsiRNA transfection induced apoptosis, inhibited proliferation and increased chemosensitivity of A549 cells to cisplatin. :Conclusion: :pSilencersurvivinsiRNA can silence survivin expression in A549 cells and subsequently inhibit proliferation, promote apoptosis, and enhance chemosensitivity of A549 cells to cisplatin. Survivin may serve as a potential target for gene therapy of lung cancer.

国家自然科学基金资助项目（No. 30371624）