目的:探讨:E1A:基因对人鼻咽癌CNE2细胞裸鼠移植瘤生长的抑制作用和对放疗的增敏作用及其相关机制。方法:以腺病毒为载体，将:E1A:基因导入CNE2细胞，获得含:E1A:的阳性克隆。通过裸鼠致瘤实验，观察:E1A:基因的抑瘤作用。观察:E1A:基因疗法及其与放疗联合应用对CNE2细胞裸鼠移植瘤的疗效。RTPCR法检测:E1A:基因治疗对:P53:基因表达的影响。结果:建立稳定转染AdE1A的CNE2阳性细胞克隆（CNE2AdE1A），CNE2AdE1A组小鼠细胞移植瘤较CNE2组和CNE2Adβgal组（稳定转染Adβgal对照质粒的CNE2细胞）成瘤时间晚、肿瘤小。单纯放疗、单纯AdE1A基因治疗及AdE1A+放疗3种治疗方法均可抑制CNE2裸鼠移植瘤的生长，抑瘤率分别为（60.32±5.34）%、（70.53±6.12）%和（97.15±4.87）%。:E1A:基因治疗能上调鼻咽癌组织中:P53:的表达。结论::E1A:可抑制人鼻咽癌细胞裸鼠移植瘤的生长并增加其对放疗的敏感性，该作用可能与:E1A:上调:P53:基因的表达有关。

Objective:To study the inhibitory effect of :E1A: gene on the growth of tumors in nude mice implanted with nasopharygeal carcinoma CNE2 cells and its promotion effect on the radiosensitivity of CNE2implanted tumors, and to investigate the related mechanism. :Methods: ::E1A: gene was transfected into CNE2 cells using adenovirus system, and stable :E1A: positive clones were established. The inhibitory effect of :E1A: on tumor formationability of CNE2 cells was observed in nude mice. The efficacy of :E1A: gene therapy with or without radiotherapy against CNE2 cellimplanted tumors was evaluated. The effect of :E1A: gene therapy on the expression of :P53: was detected by RTPCR. :Results: :CNE2 cells stably transfected with :E1A: gene (CNE2AdE1A）were successfully established. The tumor formation time was later and tumor size was smaller in CNE2AdE1A cellimplanted mice compared with those in CNE2 cell and CNE2Adβgal cellimplanted mice (CNE2 cells stably transfected with Adβgal). Radiotherapy, :E1A: gene therapy and :E1A: gene+radiotherapy all suppressed the growth of implanted tumors, with the tumor suppression rates being (60.32±5.34)%, (7053±6.12)%, and (97.15±4.87)%, respectively. :E1A:  gene therapy significantly increased the expression of :P53 : gene in tumor tissues. :Conclusion: ::E1A: can inhibit the growth of tumors in mice implanted with nasopharygeal carcinoma cells, and enhance its sensitivity to radiotherapy, which may be related to the increased expression of :P53: gene in tumor tissues.

国家自然科学基金资助项目（No. 30400118）