目的：构建胰岛素样生长因子结合蛋白7（insulinlike growth factor binding protein 7，IGFBP7）表达质粒(pEGFC1-IGFBP7)，研究IGFBP7对恶性黑素瘤细胞SK-MEL-28凋亡的影响。方法：构建pEGFC1-IGFBP7质粒，并将pEGFC1-IGFBP7质粒及空质粒分别转染入SK-MEL-28细胞，荧光显微镜观测细胞转染效率，Annexin-FITC/PI检测转染后SK-MEL-28细胞的凋亡。结果：成功构建pEGFC1IGFBP7质粒，用Effectene试剂能将pEGFC1IGFBP7质粒有效转染入SK-MEL-28细胞，转染效率达61%。流式细胞仪结果显示pEGFC1IGFBP7可明显促进SKMEL28细胞凋亡，转染24 h后的凋亡率达（28.4±257）%，转染空质粒及未转染组细胞凋亡率分别为（5.8±0.44）%和（6.4±0.71）%（F=406.138，P<0.05）。结论：pEGFC1-IGFBP7能有效诱导黑素瘤SK-MEL-28细胞凋亡，为IGFBP7为基础的黑素瘤基因治疗提供了实验依据。

Objective:To construct the insulinlike growth factor binding protein 7 (IGFBP7) expression plasmid (pEGFC1IGFBP7) and to investigate the effect of IGFBP7 on the apoptosis of SKMEL28 (human malignant melanoma cell line) cells. Methods: The pEGFC1IGFBP7 plasmid was constructed; pEGFC1IGFBP7 and empty plasmids were transfected into SKMEL28 cells separately. The transfection efficiency was observed under fluorescence microscope. Apoptosis of SKMEL28 cells after transfection was detected by AnnexinFITC/PI staining. Results:The pEGFC1IGFBP7 plasmid was successfully constructed and was effectively transfected into SKMEL28 cells by Effectene reagent, with the transfection rate being 61%. The results of flow cytometry showed that pEGFC1IGFBP7 significantly induced apoptosis of SKMEL28 cells, with the apoptotic rates of pEGFC1IGFBP7, empty vector, and nontransfected plasmid groups being (28.4±2.57)%, (5.8±0.44)%, and (6.4±0.71)% 24 h after transfection, respectively (F=406.138, P<005). Conclusion:pEGFC1IGFBP7 can effectively induce apoptosis of malignant melanoma SKMEL28 cells, which provides an experimental basis for IGFBP7 genebased therapy of malignant melanoma.

国家教育部新教师基金资助项目（No.20070487140）