目的：研究干扰素/维甲酸诱导死亡基因（retinoidinterferoninduced mortality，GRIM19）对结肠癌SW480细胞凋亡的影响。方法：构建GRIM19真核表达载体（pCMVFlagGRIM19），转染入SW480细胞中，Western blotting检测GRIM19及凋亡相关蛋白Balxl的表达，采用AnnexinV/PI和线粒体膜电位JC-1染色检测SW480细胞的凋亡。结果：成功构建pCMVFlagGRIM19真核表达载体。pCMV-Flag-GRIM-19质粒转染SW480细胞后，GRIM19的表达上调，凋亡抑制蛋白Bclxl的表达则下调。转染空质粒pCMVFlag组SW480细胞凋亡率为（7.7±1.39）%，转染pCMV-Flag-GRIM-19质粒后SW480细胞凋亡率为（15.0±2.52）%（P<0.05）。线粒体膜电位检测显示转染空质粒pCMVFlag组SW480细胞膜电位降低细胞为（7.5±2.09）%，而转染pCMVFlagGRIM19后细胞线粒体膜电位降低细胞为（17.5±3.07）%（P<0.05）。结论：GRIM-19体外转染可有效诱导结肠癌SW480细胞凋亡。

Objective: To investigate the effect of retinoidinterferoninduced mortality (GRIM-19) gene on the apoptosis of colon cancer. Methods:A GRIM19 eukaryotic expression vector (pCMV-Flag-GRIM-19) was constructed and transfected into SW480 cells. Expressions of GRIM-19 and apoptosisrelated proteins were detected by Western blotting analysis. Apoptosis of SW480 cells was measured by AnnexinV/PI assay and mitochondrial membrane potential JC-1 staining. Results:The GRIM19 eukaryotic expression vector pCMVFlagGRIM-19 was successfully constructed. Expression of GRIM19 in SW480 cells was upregulated and that of apoptosisrelated protein Bclxl was downregulated after transfection with pCMV-Flag-GRIM-19. Apoptosis rate was (7.7±1.39)% in SW480 cells transfected with pCMV-Flag empty vector and (15.0 ± 2.52)% in pCMV-Flag-GRIM-19 transfected cells (P<0.05). Mitochondrial membrane potential was decreased in (7.5±2.09)% of pCMVFlag transfected cells and (17.5±3.07)% of pCMV-Flag-GRIM-19 transfected cells (P<0.05). Conclusion:In vitro GRIM-19 transfection can effectively induce apoptosis of colon cancer SW480 cells.

国家自然科学基金资助项目（No. 30471673，30872472，30800569）；国家重点基础研究发展计划（973）项目（No.2009CB521802）