摘 要 目的：探讨不同P2X家族受体在小鼠急性T淋巴细胞白血病发展中的表达变化规律。方法：制备Notch1过表达小鼠GFP+T细胞急性淋巴细胞白血病移植模型，流式术分选CD45.2+GFP+白血病细胞，实时定量PCR检测P2X受体家族的表达变化，荧光分光光度计检测P2X7受体介导的钙离子浓度的变化。结果：对照组和白血病组小鼠骨髓细胞表达除P2X5外的其他6种P2X家族受体。Notch1过表达导致的小鼠白血病发展过程中，P2X7的表达水平逐渐升高，P2X1和P2X3的表达水平逐渐降低，而P2X2、P2X4和P2X6表达水平没有显著变化；分选后的CD45.2+GFP+白血病细胞中，P2X家族受体的表达呈现相同的规律。对照组和白血病组小鼠骨髓细胞中P2X7受体在激动剂苯甲酰-苯甲酸ATP（BzATP）的刺激下都能介导细胞内钙离子浓度升高，但白血病组小鼠骨髓细胞内钙离子处于持续高浓度状态，而对照组小鼠细胞内钙离子浓度短暂升高后逐渐下降；P2X7介导的这种钙离子反应可被其特异的拮抗剂KN62所阻断。结论：P2X受体家族中P2X1、P2X3和P2X7的表达变化与小鼠急性T淋巴细胞白血病的发展相关，提示其介导的细胞间通讯可能在白血病发展中发挥重要作用。

Abstract Objective:To investigate the expression features of P2X family receptors during the development and progression of murine acute T lymphoblastic leukemia. Methods: A Notch1 over-expressing murine T cell induced-acute lymphoblastic leukemia model was prepared. CD45.2+GFP+ leukemia cells were sorted by flow cytometry. The expressions of P2X family receptors were determined by real-time PCR. P2X7 receptor-mediated intracellular Ca 2+ change was measured by fluorescent spectrophotometer. Results: Mouse bone marrow cells (BMNCs) of both control and leukemia mice expressed all P2X receptors except for P2X5. P2X7 receptor expression increased gradually during the induction of T lymphoblastic leukemia; P2X1 and P2X3 receptors decreased; and P2X2, P2X4 and P2X6 receptors had no detectable changes. Similar expression patterns were observed in sorted CD45.2+GFP+ leukemia cells. P2X7 receptor-mediated calcium response was detected in BMNCs from both leukemia and control mice; and the response could be blocked by its specific antagonist KN62. However, the calcium response showed different patterns: it sustained an increase in leukemia group, whereas gradually decreased after reaching peak in the control group. Conclusion: P2X1, P2X3 and P2X7 receptor expressions are related to the development and progression of murine acute T lymphoblastic leukemia, suggesting that intercellular communications mediated by these receptors may play important roles in leukemia.

国家自然科学基金资助项目（No. 30570770）；天津市自然科学基金资助项目（No. 06YFJMJC15700）