摘 要 目的：研究成神经细胞瘤细胞株SK-N-SH 中Foxp3的表达及其对化疗药物环磷酰胺（cyclophosvnamide，CTX）和吡柔比星（pirarubicin, THP）的敏感性。方法：体外培养SK-N-SH细胞，流式细胞术检测Foxp3在SK-N-SH细胞中的表达。MTT法检测化疗药物CTX、THP对SK-N-SH细胞的敏感剂量；流式细胞术及real-time PCR检测CTX、THP对SK-N-SH细胞中Foxp3表达的影响。结果：流式细胞术检测结果显示，SK-N-SH细胞高表达Foxp3分子。CTX作用于SK-N-SH细胞的敏感剂量为6 mmol/L，THP作用于SK-N-SH细胞的敏感剂量为80 ng/ml。6 mmol/L CTX或80 ng/ml THP以及两者的联合不能抑制SK-N-SH细胞中Foxp3的表达（P>0.05）；real-time PCR结果也证实，CTX或THP以及两者的联合不能抑制SK-N-SH细胞中Foxp3 mRNA的表达。结论：成神经细胞瘤细胞株SK-N-SH高表达Foxp3蛋白，但其表达对化疗药物CTX和THP不敏感。

Abstract Objective: To investigate Foxp3 expression in neuroblastoma cell line SK-N-SH and its chemosensitivity to cyclophosvnamide (CTX) and pirarubicin (THP). Methods: SK-N-SH cells were cultured in vitro, and Foxp3 expression in SK-N-SH cells was examined by flow cytometry (FCM). The sensitive dosages of CTX and THP on SK-N-SH cells were determined by MTT assay. The effects of CTX or THP on Foxp3 expressions in SK-N-SH cells were examined by FCM and real-time PCR. Results: FCM results showed that SH-N-SK cells expressed high level of Foxp3. The sensitive dosage of CTX on SK-N-SH cells was 6 mmol/L, and that of THP was 80 ng/ml. CTX (6 mmol/L), THP (80 ng/ml) alone or in combination could not inhibit the expression of Foxp3 in SK-N-SH cells (P>0.05). Real-time PCR data also showed that CTX, THP alone or in combination could not down-regulate the expression of Foxp3 in SK-N-SH cells at mRNA level (P>0.05). Conclusion: Neuroblastoma SK-N-SH cells can express high level of Foxp3, but Foxp3 shows no chemosensitivity to CTX and THP.

国家高技术研究发展计划（863计划）资助项目（No.2007AA021004）