目的：研究JWA基因（又称ARL6IP5基因，ADPribosylationlike factor 6 interacting protein 5）对肿瘤细胞P糖蛋白表达及其功能的影响。方法：利用脂质体转染技术将JWAshRNA重组质粒及其空载质粒转入人绒毛膜癌细胞JAR和人乳腺癌细胞MCF7，将FlagJWA重组质粒及其空载质粒转入人绒毛膜癌耐依托泊苷(etoposide,VP16)JAR/VP16细胞；Western blotting检测JWA和P糖蛋白的表达，流式细胞术分析细胞内罗丹明123 (Rhodamine 123, Rh123)的潴留情况。结果：JWAshRNA重组质粒转入JAR细胞和MCF7细胞后，JWA表达水平降低，P糖蛋白表达水平上调，罗丹明潴留减少；FlagJWA重组质粒转入JAR/VP16细胞后，JWA表达水平上调，P糖蛋白表达水平下降，罗丹明潴留增加。结论：JWA基因可以调控肿瘤细胞P糖蛋白的表达，并影响其转运功能。

Objective:To study the effects of JWA (ADPribosylationlike factor 6 interacting protein 5, ARL6IP5) gene on expression and function of Pglycoprotein in tumor cells. Methods: JWAshRNA and controlshRNA plasmids were transfected into human choriocarcinoma cell line JAR and human breast cancer cell line MCF7,and flagJWA and flagcontrol plasmids were transfected into etoposide (VP16)resistant JAR cells (JAR/VP16) by liposomemediated transfection assay. JWA and Pglycoprotein expressions were examined by Western blotting analysis. Intercellular retention of rhodamine 123 (Rh123) was determined by FCM. Results: After JWA expression was downregulated by JWAshRNA transfection, Pglycoprotein expression was increased in JAR and MCF7 cells, and Rh123 retention was decreased. After JWA was overexpressed by flagJWA transfection, Pglycoprotein expression was decreased in JAR /VP16 cells and Rh123 retention was increased. Conclusion: JWA gene can regulate the expression and transportation of Pglycoprotein in tumor cells.

国家自然科学基金资助项目（No. 30771829，30930080）