目的： 研究骨桥蛋白（osteopontin，OPN）在乳腺癌各分子亚型中的表达及其临床意义。 方法： 收集2000年1月至2003年12 月第二军医大学长海医院的99例乳腺癌组织标本，免疫组织化学法检测ER（estrogen receptor）、PR(progesterone receptor)和HER-2（human epidermal growth factor receptor-2）的表达，并据此将乳腺癌患者分为luminal A型、luminal B型、HER-2过表达型和基底细胞样型。免疫组织化学法测定各分子亚型乳腺癌组织中OPN的表达情况，并分析OPN表达与乳腺癌患者临床病理特征的关系。 结果： 根据ER、PR和HER-2的不同表达情况，99例乳腺癌患者中luminal A型45例、luminal B型27例、HER-2过表达型14例、基底细胞样型13例。OPN在luminal A型、luminal B型、HER-2过表达型和基底细胞样型中表达率分别为22.2%、29.6%、71.4%和61.5%，HER-2过表达型和基底细胞样型乳腺癌组织中OPN表达率均高于luminal A型和luminal B型（P<0.01）。OPN低表达的乳腺癌患者生存率高于OPN高表达者，两者差异有统计学意义（P<0.01）。 结论： OPN在基底细胞样型和HER-2过表达型乳腺癌组织中高表达，其表达可作为乳腺癌预后的评估指标之一。

Objective: To study the expression of osteopontin (OPN) in breast carcinoma of different molecular subtypes and its clinical significance. Methods： A total of 99 breast cancer samples were collected from patients who were treated during Jan. 2000 to Dec. 2003 in Changhai Hospital. Expression of ER, PR and HER-2 was detected by inmmnohistochemical staining, and the samples were categorized as follows：luminal A, luminal B, HER-2 over-expression subtype, and basal-like subtype. The expression of OPN in all molecular subtypes was detected by immunohistochemical-SP method, then the correlation of OPN expression with patients’ clinical and pathological features was analyzed. Results: According to the expression of ER, PR and HER-2 in 99 breast cancer patients, there were 45 luminal A, 27 luminal B, 14 HER-2 over-expression and 13 basal-like subtypes. The positive rates of OPN in luminal A, luminal B, HER-2 over-expression and basal-like subtypes were 22.2%, 29.6%, 71.4% and 61.5%, respectively. The positive rates of OPN in HER-2 over-expression and basal-like subtypes were significantly higher than those in luminal A and luminal B subtypes (P<0.01). The survival rate of patients with low OPN expression was significantly higher than that of patients with high OPN expression (P<0.01). Conclusion: OPN is highly expressed in basal-like and HER-2 over-expression subtypes of breast cancer, which indicates that OPN may be a useful indicator for prognosis of breast carcinoma.

国家自然科学基金资助项目（No.30870975）