目的： 探讨原发性肝细胞癌（hepatocellular carcinoma,HCC）组织中免疫细胞的浸润及分布情况。 方法： 采用免疫组化S-P法，检测采集自福建省肿瘤医院30例HCC癌组织、癌周组织（距肿瘤边缘<2 cm）、非癌肝组织（距肿瘤边缘＞5 cm）石蜡标本中CD3、CD4、CD8、FoxP3、CD20、CD56、CD68的表达。 结果： 免疫组织化学结果显示，HCC患者CD3+、CD4+、FoxP3+细胞数在癌周组织中最高，癌组织中其次，非癌肝组织中最低（P<0.05）；CD8+、CD56+、CD68+细胞数在癌周组织中最高，非癌肝组织其次，癌组织中最低（P<005）；CD20+细胞数在肝癌组织、癌周组织、非癌肝组织中无明显差异（P>005）。肝癌组织中CD3+、CD4+、CD8+、CD56+、CD68+细胞主要分布于癌巢中的间质区，FoxP3+细胞呈散在分布。 结论: 肝癌组织局部微环境中，杀伤性免疫细胞减少，抑制性免疫细胞增加，从而导致局部免疫抑制状态。

Objective: To investigate the infiltration and distribution of immunocytes in primary hepatocellular carcinoma (HCC) tissues. Methods: Thirty HCC tissues were obtained from Tumor Hospital of Fujian Province. The expressions of CD3+, CD4+, CD8+, FoxP3+, CD20+, CD56+, and CD68+ cells in liver tumor tissues, liver peri-tumor tissues and non-tumor liver tissues were detected by immunohistochemistry S-P assay. Results: Immunohistochemistry results showed that the numbers of CD3+, CD4+, FoxP3+ cells were highest in liver peri-tumor tissues, less in liver tumor tissues, and least in the corresponding non-tumor liver tissues (P<0.05); the numbers of CD8+, CD56+, CD68+ cells were highest in the liver peri-tumor tissues, less in non-tumor liver tissues, and least in liver tumor tissues (P<0.05); and the CD20+ cell numbers had no significant difference between liver tumor tissues, liver peri-tumor tissues, and non-tumor liver tissues (P>005). CD3+, CD4+, CD8+, CD56+, and CD68+ cells were mainly distributed in the mesenchyma of the liver tumor tissue, and FoxP3+ cells were scattered among the whole tumor tissue. Conclusion: Cytotoxic immunocyte is decreased and the inhibitory immunocyte is increased in the microenvironment of hepatocellular carcinoma, which results in an immunosuppression status in tumor tissues.

福建省科技厅重点项目（No.2008I0012）