目的： 研究IL-2、IFN-α和IFN-γ对人肾透明细胞癌786-0细胞上B7-H1表达的影响。 方法： IL-2、IFN-α和IFN-γ刺激786-0细胞，RT-PCR检测刺激后786-0细胞B7-H1 mRNA的表达，免疫细胞化学、免疫荧光染色和流式细胞术检测刺激后786-0细胞中B7-H1蛋白的表达。 结果： RT-PCR检测结果显示，IL-2、IFN-α和IFN-γ组786-0细胞的B7-H1 mRNA表达量明显高于未经刺激的786-0细胞（0.75±0.06、0.68±0.05、0.95±0.08 vs 0.30±0.03，P﹤0.05或P<0.01）；免疫细胞化学和免疫荧光检测结果显示，B7-H1蛋白主要表达在786-0细胞的细胞膜上，IL-2、IFN-α和IFN-γ刺激后B7-H1表达明显上调；流式细胞术检测结果显示，IL-2、IFN-α和IFN-γ组786-0细胞的B7-H1分子表达阳性率明显高于未经刺激的786-0细胞\[（6570±326）%、（56.52±1.75）%、（84.05±3.52）% vs（20.49±1.03）%，P<0.01\]。 结论： IL-2、IFN-α和IFN-γ均上调786-0细胞B7-H1 mRNA和蛋白的表达，其中IFN-γ上调程度最高，IFN-α上调程度最低。

Objective : To study the effects of IL-2, IFN-α and IFN-γ on B7-H1 expression in renal clear cell carcinoma 786-0 cells. Methods: 786-0 cells were stimulated with IL-2, IFN-α and IFN-γ; the expression of B7-H1 mRNA was examined by RT-PCR; and the expression of B7-H1 protein was detected by immunocytochemistry staining, immunofluorescence staining, and flow cytometry assay. Results: RT-PCR results showed that B7-H1 mRNA expression was higher in 786-0 cells stimulated with IL-2, IFN-α, and IFN-γ compared with those in unstimulated cells (0.75±0.06,0.68±005, 0.95±0.08 vs 0.30±0.03, P﹤0.05). B7-H1 protein was distributed in the cell membrane as showed by immunocytochemistry and immunofluorescence staining, and the expression of B7-H1 protein was up-regulated after IL-2, IFN-α, and IFN-γ stimulation. Flow cytometry results showed that B7-H1 protein expressions in 786-0 cells stimulated with IL-2, IFN-α, and IFN-γ were significantly higher than that in unstimulated cells (\[65.70±3.26\]%, \[56.52±175\]%, \[84.05±3.52\]% vs \[20.49±1.03\]%, P<0.01). Conclusion: IL-2, IFN-α and IFN-γ can all up-regulate the expression of B7-H1 mRNA and protein in 786-0 cells, with IFN-γ showing the most potent up-regulation effect and IFN-α showing the least one.