目的： 研究螺旋藻硒多糖（polysaccharide from Se-enriched spirulina platensis，Se-PSP）各组分对肿瘤细胞生长的影响。 方法： 超声波法从富硒螺旋藻（Se-enriched spirulina，Se-SP）中提取Se-PSP总糖和各组分（Se-PSP1、Se-PSP2、Se-PSP3和Se-PSP4），DEAE-纤维素层析对各组分进行分离纯化。MTT法检测10 μg/ml时 Se-PSP总糖及不同组分对卵巢癌SKOV-3细胞生长的影响；观察不同质量浓度的Se-PSP2对卵巢癌SKOV-3细胞、肝癌HepG-2细胞、胃癌BGC-803细胞、鼻咽癌CNE细胞和舌鳞癌Tca-8113细胞生长的影响。 结果： Se-SP分离纯化得到4个不同组分（Se-PSP1、Se-PSP2、Se-PSP3和Se-PSP4），其中Se-PSP2质量分数最高。10 μg/ml Se-PSP总糖及其4个组分对SKOV-3细胞生长的抑制率分别为(33.393±5.437)%、(22.290±8.399)%、(44881±4.878)%、(45.354±4.931)%和(46.203±4.455)%，其中Se-PSP2、Se-PSP3和Se-PSP4抑制率显著大于Se-PSP总糖及Se-PSP1（P<0.05），但前3者无明显差别（P>0.05）。Se-PSP2能剂量依赖性抑制SKOV-3、HepG-2、BGC-803和Tca-8113肿瘤细胞的增殖（均P<0.05），抑制作用具有肿瘤广谱性。 结论： Se-PSP2是Se-PSP的主要成分，对多种肿瘤细胞的生长有抑制作用，有望成为治疗肿瘤的辅助药物。

Objective : To investigate the effects of different polysaccharides isolated from Se-enriched spirulina platensis (Se-PSP) on the growth of tumor cells. Methods: Crude total sugar Se-PSP and its different components (Se-PSP1, Se-PSP2, Se-PSP3 and Se-PSP4) were extracted from se-enriched spirulina (Se-SP) by ultrasonic method, and four Se-PSP components were further isolated and purified by DEAE-cellulose column. The effects of total sugar Se-PSP and four different components (10 μg/ml) on the growth of SKOV-3 cells, and different concentrations of Se-PSP2 on the growth of SKOV-3 cells, HepG-2 cells, BGC-803 cells, CNE cells and Tca-8113 cells were examined by MTT method. Results: Four different components (Se-PSP1, Se-PSP2, Se-PSP3 and Se-PSP4) were isolated and purified from Se-PSP, with Se-PSP2 being the most abundant one. The inhibitory rates of Se-PSP and four different components (Se-PSP1, Se-PSP2, Se-PSP3 and Se-PSP4) on growth of SKOV-3 cells were (33.393±5.437)%, (22.290±8.399)%, (44.881±4.878)%, (45.354±4931)% and (46.203±4.455) %, respectively, with the inhibitory rates of Se-PSP2, Se-PSP3 and Se-PSP4 being significantly higher than those of Se-PSP and Se-PSP1 (P<0.05), but those of Se-PSP2, Se-PSP3 and Se-PSP4 showing no difference (P>0.05). Se-PSP2 dose-dependently inhibited the proliferation of SKOV-3, HepG-2, BGC-803 and Tca-8113 cells (all P<0.05), and showed broad anti-tumor spectra. Conclusion: Se-PSP2 is the main component of Se-PSP, and it can inhibit the growth of various types of tumor cells, making it a promising adjuvant drug for treatment of malignant tumors.

广西研究生教育创新计划资助项目（No. 2007105981002M07）