目的：研究消癌解毒方（ Xiaoai Jiedu Recipe, XJR）对肝癌H22细胞小鼠移植瘤的抑制作用及其可能的机制。方法：建立小鼠H22移植瘤模型，分对照组和XJR低、中、高剂量（10、30、90 g/kg）组及顺铂(0.001 g/kg)组进行治疗，检测各组移植瘤生长情况,H-E染色观察各组移植瘤组织的病理改变。流式细胞术检测各组移植瘤细胞周期及凋亡率，ELISA法测定各组移植瘤小鼠外周血VEGF水平。结果：与对照组比较，XJR各剂量以及顺铂对H22移植瘤的生长均具有显著的抑制作用，抑瘤率分别为24.5%、42.8%、21.1%、58.6% (P<0.05或P<0.01）。病理观察见中剂量XJP组和顺铂组移植瘤组织大片状坏死，有明显染色质固缩环。中剂量XJR和顺铂将移植瘤组织细胞阻滞于G0/G1期（P<0.05），S期细胞数明显减少（P<0.05）。中剂量XJR组移植瘤细胞的凋亡率与顺铂组相当\[（60.52±6.40)% vs (71.32±16.02)%，P>0.05\]。中、高剂量XJR组和顺铂组小鼠外周血VEGF水平均显著降低\[（104.3±6.1)、(105.8±7.2)、(88.6±4.3) vs (120.7±12.6) ng/ml，P<0.05或P<0.01\]。结论：XJR能抑制H22小鼠移植瘤的生长，促进移植瘤细胞凋亡、抑制VEGF的产生可能是其抗癌作用机制之一。

Objective:To study the inhibitory effects of Xiaoai Jiedu Recipe(XJR) against transplanted hepatocarcinoma H22 tumors in mice and the related mechanism. Methods: H22-transplanted tumor mouse models were established and were divided into control group, XJR treatment groups (10, 30, 90 g/kg), and cisplatin treatment group. The growth of tumors in different groups was measured, and pathology changes of transplanted tumor tissues in different groups were examined by H-E staining. Flow cytometry was applied to examine cell cycle and sub-diploid apoptosis rate of transplanted tumor cells, and VEGF levels in the peripheral blood of mice were measured by ELISA. Results: Compared with control group, different XJR groups and the cisplatin group significantly inhibited growth of H22-transplanted tumors (245%, 42.8%, 21.1, 58.6% vs 0, P<0.05, P<0.01), cells in XJR groups showing massive necrosis and obvious chromosome condensation. Medium-dosage XJR and cisplatin blocked transplanted tumor cells in G0/G1 phase (P<005), and the cells in S phase were significantly reduced (P<0.05). The sub-diploid apoptosis rate of transplanted tumor cells in medium-doseage XJR group was similar to that in cisplatin group (60.52±6.40 vs 71.32±16.02, P>0.05). Peripheral VEGF levels were significantly lower in the medium-, large-dosage XJR groups and cisplatin group than in the control group (104.3±6.1, 105.8±7.2, 88.6±4.3 vs 120.7±12.6, P<0.05, P<0.01). Conclusion: Xiaoai Jiedu Recipe can inhibit the growth and promote apoptosis of H22-transplanted tumor cells, and inhibition of VEGF may be one of the mechanisms for the anticancer effect.

国家自然科学基金资助项目（No. 30973742/C190201）；江苏省自然科学基金资助项目（No. BK2007240）；江苏省高校自然科学研究面上项目资助（No. 09KJB360003）；江苏省普通高校研究生科研创新项目资助（No. CX09S_037Z）；江苏省中医药局课题（No. LB09032）