目的：探讨和厚朴酚（honokiol，HNK）对人急性髓性白血病 KG1a细胞凋亡的影响及其可能的机制。方法: XTT法检测不同质量浓度HNK对KG1a细胞增殖的影响，流式细胞术检测不同质量浓度HNK作用后KG1a细胞的细胞周期及凋亡，RT-PCR法检测KG1a细胞〖STBX〗Bcl-2、Bid、Bax、Bak、Bad、P53、NF-κB〖STBZ〗等凋亡相关基因的表达。结果：HNK（2.5、5、8、10、15、20、40 μg/ml）对KG1a细胞的增殖有明显抑制作用，且呈时间和剂量依赖性（P<0.01），其中24、48 h的半数抑制浓度（IC50）分别为10.23、8.25 μg/ml。流式细胞术结果显示，经HNK（5、10 μg/ml）处理后，KG1a细胞被阻滞在G0/G1期，早期凋亡率分别为（11.16±1.27）%和（21.46±3.13）%，显著高于对照组的（6.03±1.10）%（P<0.01）。RT-PCR检测结果显示，HNK（10 μg/ml）处理后KG1a细胞内促凋亡基因Bax表达显著上调，Bad轻度上调；抗凋亡基因NF-κB表达显著下调。结论：HNK能诱导人急性髓性白血病 KG1a细胞凋亡，其机制可能与Bax、Bad基因表达上调及NF-κB基因表达下调有关。

Objective:To investigate the apoptosis-inducing effect of honokiol (HNK) on human acute myeloid leukemia KG1a cells and the possible mechanism. Methods: KG1a cells were treated with different concentrations of HNK, and then the proliferation of KG1a cells was detected by XTT assay. Flow cytometry was performed to examine the cell cycle and apoptosis of KG1a cells after HNK (0, 5, 10 μg/ml) treatment. RT-PCR technique was used to detect the expression of apoptosis-related genes (〖STBX〗Bcl-2, Bid, Bax, Bak, Bad, P53 and NF-κB〖STBZ〗) in KG1a cells. Results: HNK (2.5, 5, 8, 10, 15, 20, and 40 μg/ml) significantly inhibited the growth of KG1a cells in a time- and dose-dependent manner (P<0.01), and IC50 concentrations in 24 h, 48 h were 10.23 μg/ml and 8.25 μg/ml, respectively. Flow cytometry results revealed that KG1a cells were arrested at G0/G1 phase after treated with HNK; the early apoptotic rates of KG1a cells after HNK treatments (5 μg/ml and 10 μg/ml) were significantly higher than those in control group (0 μg/ml), with apoptotic rates being (\[11.16±1.27\]%, \[21.46±3.13\]% vs \[6.03±1.10\]%, P<0.01). Meanwhile, RT-PCR revealed the mRNA expression of apoptosis-promoting gene Bax was significantly increased, with Bad slightly improved after HNK treatments compared with control group; meantime, the apoptosis-inhibiting gene (NF-κB) was markedly decreased. Conclusion: HNK can induce apoptosis of KG1a cells, which might be related to increased expression of Bax, Bad genes, and decreased expression of NF-κB gene.

国家自然科学基金资助项目 （No.30973454）