目的：观察松生拟层孔菌（Fomitopsis pinicola, FP）乙醇提取物Ⅰ号（简称FP-Ⅰ）的体内外抗肿瘤活性及其可能的机制。方法：以乙醇提取FP ，获取乙酸乙酯相即为FP-Ⅰ。MTT法检测FP-I体外对小鼠肝癌细胞株H22及小鼠肉瘤细胞株S180增殖的抑制作用；建立S180细胞小鼠移植瘤模型，检测各组小鼠瘤质量、抑瘤率、胸腺指数、脾脏指数、外周血白细胞数及淋巴细胞比例、肿瘤红细胞花环形成率，H-E染色观察FP-Ⅰ对各组S180移植瘤细胞凋亡的影响及心、肝、肾、胸腺和脾脏的组织学变化。结果：50、100、200、400 μg/ml FP-Ⅰ对S180细胞增殖的抑制率分别为22.35%、32.49%、40.01%和74.01%，对H22细胞的抑制率分别为45.19%、51.10%、66.37%和82.40%。 25、50、100 mg/kg FP-Ⅰ对小鼠S180移植瘤生长的抑瘤率分别为79.92%、66.18%和78.45%，CTX阳性对照（30 mg/kg）的抑瘤率为84.10%；中、高剂量FP-Ⅰ组S180荷瘤小鼠的淋巴细胞比例、胸腺指数及红细胞花环率均有一定程度的提高（P<0.05或P<0.01）；各剂量组的S180移植瘤细胞均出现细胞凋亡的典型形态变化。结论：松生拟层孔菌提取物具有较强的抗肿瘤活性，其机制与其增强小鼠免疫功能和诱导肿瘤细胞凋亡有关。

Objective:To study the in vitro and in vivo anti-tumor activities of Fomitopsis pinicola extract and their possible mechanisms. Methods: Fomitopsis pinicola was extracted by alcohol and the resulting acid ethyl esters extract was named FP-I. The inhibitory effects of FP-I on proliferation of mouse hepatocellular carcinoma H22 cells and mouse sarcoma S180 cells were detected by MTT assay in vitro. S180-implanted mouse model was established, and the tumor weight, tumor inhibitory rate, thymus index, spleen index, peripheral leukocyte number, peripheral lymphocyte rate, and associate tumor erythocyte rosette rate (ATER) were examined in different group. Effect of FP-I on apoptosis of S180-implanted tumor cells and the histological changes of the heart, liver, kidney, thymus and spleen were observed by H-E staining. Results: The inhibitory rates of FP-I (50, 100, 200, 400 μg/ml) on proliferation of S180 cells were 22.35%, 32.49%, 40.01%, and 74.01%, respectively; and those on H22 cells were 45.19%, 51.10%, 66.37%, and 82.40%, rspectively. The inhibitory rates of FP-I (25, 50, 100 μg/ml) on growth of S180-implanted tumors were 79.92%, 66.18% and 78.45%, respectively, and that of CTX (30 mg/kg) positive control was 84.10%. Proportion of lymphocyte in periphoral blood, thymus index and ATER of S180-bearing mice were significantly increased in both the high-and medium-dose FP-I groups (P<0.05 or P<0.01). S180-implanted tumor cells in all FP-I treatment groups showed typical apoptotic morphology. Conclusion: Fomitopsis pinicola extract has anti-tumor activity, which might relate to its ability to improve the immune function of mice and to induce apoptosis of tumor cells.

湖南省教育厅科研基金资助项目（No. 08C142，No.2009-320-372）