目的：探讨凋亡抑制蛋白survivin在胰腺癌细胞株PaTu8988中的表达，及其与吉西他滨（gemcitabine，GEM）耐药的关系。方法：MTT法检测GEM（0.01、0.1、1.0、2.5、5.0、10.0 μg/ml）对PaTu8988细胞生长的抑制作用，流式细胞术检测GEM作用后PaTu8988细胞的凋亡率，RT-PCR检测survivin mRNA在PaTu8988细胞中的表达。结果：高质量浓度GEM（≥1.0 μg/ml）可显著抑制PaTu8988细胞的增殖、促进PaTu8988细胞的凋亡；而低质量浓度GEM（0.01、0.1 μg/ml）作用不明显。低质量浓度GEM时间依赖性地上调PaTu8988细胞中survivin mRNA的表达；而质量高浓度GEM作用PaTu8988细胞后，survivin mRNA的表达48 h时逐渐下降，而后逐渐上升。结论：胰腺癌PaTu8988细胞中survivin mRNA高表达，可能是其对GEM产生耐药的原因之一。

Objective:To explore the expression of apoptotic inhibitory protein survivin in pancreatic cancer cell line PaTu8988, and to study its role in the drug resistance of PaTu8988 cells to gemcitabine (GEM). Methods: The inhibitory effect of GEM (0.01, 0.1, 1.0, 2.5, 5.0, and 10.0 μg/ml) on PaTu8988 cells was detected by MTT assay; apoptosis rate of PaTu8988 cells treated with GEM was determined by flow cytometry; and the survivin mRNA expression in PaTu8988 cells was examined by RT-PCR. Results: High dosage of GEM (≥1.0 μg/ml) greatly inhibited growth and promoted apoptosis of PaTu8988 cells, while low dosage of GEM (0.01, 0.1 μg/ml) showed no effects. Low dose of GEM time-dependently increased expression of survinin mRNA in PaTu8988 cells; high dosage of GEM gradually inhibited survivin mRNA expression within the first 48 h, and then survivin mRNA expression gradually increased as time went by. Conclusion: Survivin mRNA is highly expressed in pancreatic cancer cell line PaTu8988, which may be one of the reasons for drug resistance to GEM.

南京医科大学医学重点科技发展项目（No. 08NMUZ042）