目的：探讨14-3-3σ和cyclin B1蛋白在乳腺癌组织中的表达及其与乳腺癌临床病理特征之间的关系。方法: 选择2006年11月至2009年11月在辽宁医学院附属第一医院手术治疗的乳腺癌患者95例乳腺癌和30例癌旁组织（距癌边缘5 cm）的标本，患者年龄为31～85岁，中位年龄58岁。免疫组织化学PV法和Western blotting检测14-3-3σ和cyclin B1蛋白在乳腺癌和癌旁组织中的表达，分析两者与乳腺癌临床病理特征之间的关系以及两者的相关性。结果: Western blotting与免疫组化实验结果显示，14-3-3σ蛋白在乳腺癌中表达率为22.1% (21/95)，显著低于癌旁组织的93.3%（28/30）（P<0.01）；14-3-3σ蛋白的表达与乳腺癌组织学分级、TNM分期、淋巴结转移有关（P<005）。Cyclin B1蛋白在乳腺癌中的表达率为69.5%（66/95），显著高于癌旁组织的40%（12/30）（P<0.05）；Cyclin B1蛋白的表达与乳腺癌淋巴结转移相关（P<0.05）。乳腺癌组织中14-3-3σ和cyclin B1蛋白的表达呈负相关（r=-0.333，P=0001）。结论：14-3-3σ和cyclin B1蛋白与乳腺癌的发生、转移相关，两者联合检测可为乳腺癌诊断和治疗提供参考。

Objective:To study the expressions of 14-3-3σ and cyclin B1 proteins in breast carcinoma tissues and to analyze their relationships with clinical characteristics of breast carcinoma. Methods: Ninety-five breast carcinoma tissues and thirty corresponding adjacent tissues (patients were diagnosed in First Affiliated Hospital of Liaoning Medical University from Dec. 2006 to Dec. 2009; 31〖KG-*2〗〖DK〗-85 years old, median age 58 years) were included in the present study. The expressions of 14-3-3σ and cyclin B1 proteins in the breast carcinoma tissues and adjacent tissues were detected by immunohistochemistry PV and Western blotting methods, and their correlation with clinical characteristics of breast carcinoma was analyzed. Results: Immunohistochemistry and Western blotting results showed that the expression rate of 14-3-3σ in breast carcinoma was 22.1% (21/95), which was significantly lower than that in the adjacent tissues (93.3%, 28/30; P<005); the expression of 14-3-3σ was correlated with the histological grade, TNM stage, and lymph node metastasis of breast carcinoma (P<0.05). The expression rates of cyclin B1 in breast carcinoma was 69.5% (66/95), being significantly higher than that in the adjacent tissues (40%, 12/30; P<0.05). The expression of cyclin B1 was only correlated with lymph node metastasis of breast carcinoma (P<005); expression of 14-3-3σ was negatively correlated with cyclin B1 in the breast carcinoma tissues (r=-0.333, P=0.001). Conclusion: 14-3-3σ and cyclin B1 proteins are related to the progression and metastasis of breast carcinoma, and their combined detection may help the diagnosis and treatment of breast carcinoma.

辽宁省科学技术计划项目资助（No. 2010225034），辽宁省教育厅资助项目（No. 2009A451）