目的：探讨人反义端粒酶RNA（human telomerase RNA，hTR）对肝癌细胞系BEL-7402黏附和侵袭的影响及其机制。方法：利用前期实验成功转染端粒酶正、反义RNA基因的肝癌细胞，分为正义转染组（BEL-7402-hTR-EcoRⅠ细胞）、反义转染组（BEL-7402-hTR-BamH Ⅰ细胞），空白对照组（BEL-7402细胞）。黏附实验和侵袭实验检测反义hTR转染后BEL-7402细胞的黏附和侵袭能力，免疫细胞化学检测整合素β1（integrin β1，INTβ1）、上皮钙黏蛋白（epithelial cadherin，E-cad）的表达水平，明胶酶谱法检测基质金属蛋白酶（matrix metalloproteinase，MMP）-2、-9的活性变化。结果：与空白对照组和正义hTR转染组相比，反义hTR转染组BEL-7402-hTR-BamHⅠ细胞的黏附、侵袭能力明显减低（0.204±0.029 vs 0.505±0.037、0465±0.029，34.80±3.19 vs 71.47±5.15、69.87±3.11，均P<0.05），INTβ1表达降低（0.153±0.016 vs 0.385±0.008、0375±0.014，P<0.05），E-cad表达增强（0.209±0.020 vs 0.124±0.018、0.134±0.016，P<0.05），MMP-2和MMP-9的活性受到明显抑制（2 054.22±138.52 vs 3 105.56±329.60、2 923.22±269.08，846.33±104.66 vs 1 538.89±122.85、1 453.33±126.35，均P<0.05）。结论：人反义端粒酶RNA能明显减低肝癌BEL-7402细胞的黏附、侵袭能力，其机制可能与上调E-cad的表达、下调INTβ1的表达，并抑制MMP-2和MMP-9的活性有关。

Objective:To study the effect of antisense human telomerase RNA (hTR) on adhesion and invasion of hepatocellular carcinoma (HCC) cell line BEL-7402 and its mechanism. Methods: Our previous research had successfully transfected sense or antisense hTR gene into BEL-7402 cells. This study they were divided into 3 groups: the sense hTR transfected group (BEL-7402-hTR-EcoRI), the antisense hTR transfected group (BEL-7402-hTR-BamHI) and the blank control group (BEL-7402). The adhesion and invasion of BEL-7402 cells after antisense hTR transfection were observed with adhesion and invasive assay, respectively. The expressions of integrin β1 (INTβ1) and epithelial cadherin (E-cad) were detected by immunocytochemistry, and galatin zymography was used to measure the activities of matrix metalloproteinase-2 (MMP-2) and MMP-9. Results: The adhesion and invasion abilities of antisense hTR transfected BEL-7402 cells were significantly weaker than those of the blank control and sense hTR transfected groups (0.204±0.029 vs 0.505±0037, 0.465±0.029; 34.80±3.19 vs 71.47±5.15, 69.87±3.11; all P<0.05). Meanwhile, the expression of INTβ1 was also significantly decreased (0.153 3±0.015 6 vs 0.385±0.008, 0.375±0.014 4, P<0.05), and E-cad was significantly increased (0.209±0.020 vs 0.124±0.018, 0.134±0.016; P<0.05) in the antisense hTR transfected group. The activities of MMP-2 and MMP-9 were significantly inhibited in the antisense hTR transfected group (2 05422±13852 vs 3 105.56±329.60, 2 923.22±269.08; 846.33±104.66 vs 1 538.89±122.85, 1 453.33±126.35; all P<0.05). Conclusion:Antisense hTR can inhibit the adhesion and invasion of hepatocellular carcinoma BEL-7402 cells, which may be related to the increase of E-cad expression, decrease of INTβ1 expression, and decrease of MMP-2 and MMP-9 activities.

山东省医药卫生科研项目（No. 2001CA1DBA3）