目的：以大规模平行测序技术（massively parallel signature sequencing，MPSS）筛选人肾细胞癌（renal cell carcinoma，RCC）和癌旁组织中差异表达的microRNAs（miRNAs），并验证差异表达miRNAs之一的miR-660在人RCC中的表达。方法：MPSS检测10例RCC组织及相应癌旁组织中miRNAs的表达，筛选RCC组织中差异表达miRNAs。RT-PCR检测5例RCC与癌旁组织中miR-660的表达，qPCR检测40例RCC与癌旁组织中miR-660的表达。结果：MPSS结果显示，RCC组织中283个miRNAs表达下调，187个表达上调，其中miR-660在RCC组织中表达量是癌旁组织的17.5%。RT-PCR初步验证了此结果；qPCR验证结果显示，40例RCC组织中33例miR-660表达显著低于癌旁组织，RCC组织中miR-660平均表达量是癌旁的195%。结论：人RCC组织中283个miRNAs表达下调、187个上调。miR-660在RCC组织中低表达，有可能成为RCC诊断及治疗的新靶点。

Objective:To screen differentially expressed microRNAs (miRNAs) in tumor and the corresponding paracancerous tissues of renal cell carcinoma (RCC) by massively parallel signature sequencing (MPSS), and to identify the expression of miR-660 in RCC. Methods: The expression of miRNAs in tumor and the corresponding paracancerous tissues of 10 RCC patients was examined by MPSS, and the differentially expressed miRNAs were screened. Expression of miR-660 in tumor tissues of 5 patients and the paracancerous tissues of 40 RCC patients were further examined by RT-PCR and qPCR, respectively. Results: The MPSS results showed that 283 miRNAs were up-regulated and 187 were down-regulated in RCC tissues, and the expression level of miR-660 in RCC tissues was only 19.5% of that in the paracancerous tissues. The MPSS results were primarily confirmed by RT-PCR, and qPCR results further showed that miR-660 expression in 34 of 40 RCC tissues was significantly lower than that in the paracancerous tissues, with the average level being only 11.7% of that in the paracancerous tissues. Conclusion: A total of 283 miRNAs are up-regulated and 187 down-regulated in RCC tissues; and miR-660 is lowly expressed in RCC, which may be a new target for the diagnosis and treatment of RCC.

国家自然科学基金资助项目（No. 30900817）；深港创新圈项目资助（No. ZYB200907080110A）