目的：探讨胃癌患者外周血和肿瘤组织中CD14+DRlow/- 髓源抑制性细胞（myeloid-derived suppressor cells，MDSCs）的表达及其与肿瘤病理特征的关系。方法：选取2009年3月至2010年10月安徽医科大学第三附属医院胃癌患者43例（Ⅰ期9例、Ⅱ期13例、Ⅲ期14例、Ⅳ期7例），另采集26例正常健康者作为对照组。流式细胞术检测外周血、瘤组织中CD14+ DRlow/-MDSCs的表达水平，分析MDSCs表达水平与肿瘤病理特征的相关性。结果：胃癌患者肿瘤组织中CD14+ DRlow/-MDSCs的表达显著高于自身外周和健康对照者外周血的表达水平\[2.87±1.93)% vs (2.37±1.7)%, (0.89±0.47)%,P<005和P<0.01\]，后两者间差异也有统计学意义(P<0.01)。CD14+DRlow/-MDSCs的表达与胃癌的恶性程度呈正相关，晚期胃癌组织内CD14+ DRlow/-MDSCs表达明显增加（Ⅰ∶Ⅱ∶Ⅲ∶Ⅳ=(1.15±0.78)%∶(1.71±0.92)%∶(2.25±1.24)%∶(4.85±2.37)%, P＜0.05）。同时，肿瘤浸润组织与非浸润组织的CD14+ DRlow/-MDSCs表达也有明显差异\[3.90±1.67)% vs (262±1.53)%，P<0.05\]。结论：CD14+ DRlow/-MDSCs在胃癌患者外周血和肿瘤组织中均高表达，与胃癌的发生、发展密切相关。

Objective:To investigate the expression of CD14+DRlow/- myeloid-derived suppressor cells (MDSCs) in peripheral blood and tumor tissues of gastric carcinoma (GC) patients and its relationship with clinicopathological of GC. Methods: Forty-three stomach carcinoma patients (9 stageⅠ, 13 stageⅡ, 14 stageⅢ, 7stageⅣ) were selected from Third Affiliated Hospital of Anhui Medical University (Mar. 2009 to Oct. 2010), and 26 healthy volunteers were used as control. CD14+DRlow/-MDSCs expression in peripheral blood and gastric carcinoma tissues was detected by flow cytometry, and its relationship with clinicopathological of GC was analyzed. Results: CD14+DRlow/-MDSCs expression in GC tissues was significantly higher than those in peripheral blood of GC patients and healthy controls (\[2.87±1.93\]% vs \[237±1.7\]%, \[0.89±0.47\]%, P<0.05 and P<0.01), and CD14+DRlow/-MDSCs expression in peripheral blood of GC patients was also higher than that in healthy controls (P<0.01). CD14+DRlow/-MDSCs expression in GC tissues was positively correlated with their malignancy stage and significantly increased in advanced GC (stage Ⅰ∶Ⅱ∶Ⅲ∶Ⅳ=(1.15±0.78)%∶(1.71±0.92)%∶(2.25±1.24)%∶(4.85±2.37)%, P<0.05). Meanwhile, CD14+DRlow/-MDSCs expression in tumor infiltration tissues was significantly higher than that in tumor un-infiltration tissues (\[3.90±167)% vs \[2.62±1.53\]%, P<0.05). Conclusion: CD14+DRlow/-MDSCs are highly expressed in peripheral blood and GC tissues, and relates to the development and progression of GC.

国家“十一五”科技重大专项（No. 2009ZX10004-309）