目的： 研究携带细胞穿膜肽11R和P53的溶瘤腺病毒SG7605-11R-P53对肝癌细胞的体外杀伤作用。 方法: 以本课题组前期实验构建的携细胞穿膜肽11R和P53的溶瘤腺病毒SG7605-11R-P53和不携11R的溶瘤腺病毒SG7605-P53感染肝癌细胞HepG2、SMMC-7721、Hep3B、Huh7和正常成纤维细胞株BJ，Western blotting检测感染后细胞P53和11R-P53的表达情况， TCID50法检测SG7605-11R-P53和SG7605-P53在肝癌细胞中的增殖能力，MTT法检测SG7605-11R-P53对肝癌细胞及正常细胞的杀伤作用。 结果： SG7605-11R-P53和SG7605-P53能在肝癌细胞中高表达P53和11R-P53蛋白。SG7605-11R-P53可在HepG2、SMMC-7721、Hep3B和Huh7细胞中大量增殖，其增殖倍数是SG7605-P53的10～100倍，但在正常BJ细胞内几乎不增殖。SG7605-11R-P53在MOI=0.1时对Hep3B细胞的杀伤率达90%，对于正常BJ细胞只有当MOI=50时才有很弱的抑制作用；SG7605-11R-P53对4种肝癌细胞杀伤作用的大小依次为Hep3B、HepG2、Huh7和SMMC-7721细胞。 结论： 携带细胞穿膜肽11R和P53的SG7605-11R-P53溶瘤腺病毒体外对4种肝癌细胞株均有较好的靶向杀伤作用，尤其对Hep3B细胞的杀伤作用最强。

Objective : To study the cytotoxicity of oncolytic adenovirus SG7605-11R-P53 containing cell-penetrating peptide (11R) on hepatocellular carcinoma cells Hep3B and Huh7 in vitro. Methods: Western blotting analysis was used to detect the expression levels of P53 and 11R-P53 in hepatocellular carcinoma cell lines HepG2, SMMC-7721, Hep3B and Huh7, and normal cell line BJ after SG7605-11R-P53 and SG7605-P53 infection. TCID50 assay was used to evaluate the replication ability of SG7605-11R-P53 and SG7605-P53 in hepatocellular carcinoma cell lines; the cytotoxicity of SG7605-11R-P53 on hepatocellular carcinoma cell lines and normal cell line was evaluated by MTT assay. Results: P53 and 11R-P53 proteins were highly expressed in both SG7605-11R-P53 and SG7605-P53 infected hepatocellular carcinoma cell lines. SG7605-11R-P53 replicated in HepG2, SMMC-7721, Hep3B and Huh7 cells but could hardly replicate in normal BJ cells, and SG7605-11R-P53 had a 10-100 times higher replication than SG7605-P53. When MOI=1, the cytotoxicity rate of SG7605-11R-P53 against Hep3B cells was 90%, and when MOI increased to 50, SG7605-11R-P53 only had a weak inhibitory effect against normal BJ cells. The cytotoxicity effect of SG7605-11R-P53 against Hep3B, HepG2, Huh7 and SMMC-7721 cells decreased gradually. Conclusion: Adenovirus SG7605-11R-P53 containing cell-penetrating peptide (11R) can efficiently kill the 4 hepatocellular carcinoma cell lines in vitro, with the strongest effect seen on Hep3B cells. This study lays a foundation for further investigating the effect of SG7605-11R-P53 against liver cancer in vivo.

国家新药创制重大专项课题（No. 2009ZX09102-235）