目的： 检测急性白血病患者外周血中Treg细胞与Th17细胞的比例以及相关细胞因子如IL-17、IL-6、TGF-β的变化，分析其相关性。 方法： 选取兰州大学第二医院血液科急性白血病初诊患者15例，另取15名健康志愿者为对照。流式细胞术检测外周血中CD3+CD4+TIL-17+辅助性T细胞（Th17细胞）、CD4+TCD25+Foxp3+调节性T细胞（Treg细胞）占CD4+T细胞的比例，ELISA法检测血清中细胞因子IL-17、TGF-β、IL-6的水平。 结果： 急性白血病患者外周血中Th17细胞占CD4+T细胞的（1.39±0.24）%，高于对照组的（0.26±0.11）%（P<0.05）；急性白血病患者外周血Treg细胞占CD4+T细胞的（1158±2.17）%，高于对照组的（2.47±0.72）%（P<0.05）；且Treg细胞与Th17细胞呈正相关（γ=0.37)。急性白血病患者血清中TGF-β、IL-6、IL-17的水平分别为（26.06±2.43）、（14.66±2.47）、（18.63±2.38）pg/ml，高于对照组的（13.41±192）、（1.44±0.29）、（10.34±1.71）pg/ml（均P<0.05）。 结论： 急性白血病患者外周血中Treg、Th17细胞比例升高，且两者呈正相关；急性白血病患者血清中TGF-β、IL-6、IL-17水平升高，可能影响Treg与Th17细胞的平衡。

Objective : To observe the proportions of CD3+CD4+TIL-17+ helper T cells (Th17 cells) and CD4+TCD25+Foxp3+ regulatory T cells (Tregs) in the peripheral blood of acute leukemia patients and the changes of related cytokines (including IL-17, TGF-β, IL-6), and to analyze their relationship. Methods: The ratios of Treg and Th17 cells in peripheral CD4+T cells were examined by flow cytometry. The serum levels of IL-17, TGF-β and IL-6 were measured by ELISA. Results: The ratio of Th17 cells in CD4+T cells was (1.39±0.24)%, which was significantly higher than that in the control group (\[0.26±0.11\]%, P<0.05); the ratio of Treg cells in CD4+T cells was (11.58±217)%, which was significantly higher than that in the control group (\[2.47±0.72\]%, P<0.05); and Treg cells were positively related with Th17 cells. The serum levels of TGF-β, IL-6, and IL-17 in acute leukemia patients were (26.06±243), (14.66±2.47), and (18.63±2.38) pg/ml, respectively, which were significantly higher than those in the control group (\[13.41±1.92\],\[1.44±0.29\], and \[10.34±1.71\] pg/ml, P<0.05). Conclusion: The ratios of Treg and Th17 cells are increased in the peripheral blood of acute leukemia patients, and they are positively correlated with each other. The serum levels of TGF-β, IL-6, and IL-17 are also elevated, which may affect the balance between Treg cells and Th17 cells.

甘肃省科技支撑计划资助项目（No.0804NKCA115）