目的：探讨鸦胆子油乳在体外对人大细胞肺癌NCIH460细胞的抑制作用及其机制。方法：以不同质量浓度鸦胆子油乳（2.5、5.0、10、20、40、80 μg/ml）处理NCIH460细胞，24、48、72 h后收集细胞，MTT法检测鸦胆子油乳对NCIH460细胞增殖的抑制作用；吉姆萨染色后光学显微镜下观察NCIH460细胞形态学改变；流式细胞仪测定NCIH460细胞凋亡率；caspase3酶活性试剂盒检测NCIH460细胞caspase3酶活性。结果：鸦胆子油乳可剂量和时间依赖性抑制NCIH460细胞的增殖，80 μg/ml 鸦胆子油乳作用72 h时的抑制率达（91.07±1.60）%。鸦胆子油乳作用后，NCIH460细胞呈现典型凋亡形态学改变；流式细胞术检测结果显示，10、20和40 μg/ml鸦胆子油乳作用48 h后，NCIH460细胞凋亡率分别为（45.23±430）%、（54.14±3.09）%和（61.57±7.28）%（P＜0.01）。鸦胆子油乳可剂量依赖性上调NCIH460细胞caspase3酶活性，40 μg/ml鸦胆子油乳时caspase3酶活性为（1.07±0.07）μmol/μg，是对照组的4.97倍（P＜0.01）。结论：鸦胆子油乳可能通过活化caspase3诱导NCIH460细胞凋亡，抑制NCIH460细胞增殖。

Objective: To investigate the inhibitory effect of Brucea javanica oil emulsion on the growth of human largecell lung cancer NCIH460 cells in vitro and its mechanisms. Methods: NCIH460 cells were treated with different concentration of Brucea javanica oil emulsion (2.5, 5.0, 10, 20, 40 and 80 μg/ml) for 24, 48, and 72 h. The inhibitory effects of Brucea javanica oil emulsion on proliferation of NCIH460 cells was examined by MTT; morphological changes was observed by optical microscope after Giemsa staining; apoptotic rate of NCIH460 cells was examined by flow cytometry; and caspase3 activity in NCIH460 cells was detected with caspase3 activity kit. Results: Brucea javanica oil emulsion significantly inhibited the proliferation of NCIH460 in a dose and timedependent manner, and the inhibiting rate reached (91.07±1.60)% after 80 μg/ml Brucea javanica oil emulsion treatment for 72 h. Typical apoptotic morphological changes also appeared after Brucea javanica oil emulsion treatment. Flow cytometry results demonstrated that the apoptotic rates of NCIH460 cells were (45.23±4.30)%, (54.14±3.09)% and (61.57±7.28)% after 10, 20 and 40 μg/ml Brucea javanica oil emulsion treatment for 48 h, respectively (P＜0.01). In NCIH460 cells, the Brucea javanica oil emulsion increased the activity of caspase3 in a dosedependent manner. after 40 μg/ml Brucea javanica oil emulsion treatment, caspase 3 activity reached 1.07±0.07 μmol/μg, which was 4.97 fold higher than control group (P＜0.01). Conclusion:Brucea javanica oil emulsion can induce apoptosis and inhibit the proliferation of NCIH460 cells, possibly through increasing caspase3 activity.

国家自然科学基金资助项目（No. 30670916）；沈阳市科技局科技计划资助项目(No. 1063315100)