目的：研究乳腺癌组织中Foxp3+细胞浸润和Foxp3蛋白的表达及其临床意义。方法：160例乳腺癌以及22例癌旁正常乳腺组织取自上海市黄浦区中心医院（2005年1-12月），免疫组化检测乳腺癌组织和癌旁组织中Foxp3+淋巴细胞浸润和Foxp3蛋白的表达，分析它们与乳腺癌患者病理特征以及ER、PR、P53、Bcl2、HER2表达间的关系。结果：乳腺癌间质Foxp3+细胞浸润和实质Foxp3蛋白表达阳性率均高于癌旁组织（50.6% vs 13.6%，P<0.05；38.1% vs 4.5%，P<0.05）；乳腺癌Foxp3+细胞浸润和Foxp3蛋白表达间无相关性（P>0.05）。乳腺癌间质Foxp3+细胞浸润与乳腺癌淋巴结转移、组织学分级、P53过表达呈正相关（P<0.05）；癌实质Foxp3蛋白表达与肿瘤淋巴结转移、组织学分级、pTNM和P53过表达呈正相关（P<0.05）；但两者与ER、PR、HER2、Bcl2过表达均无关（P>0.05）。单因素分析提示，乳腺癌中Foxp3表达和Foxp3+细胞浸润都是影响5年生存率的因素；多因素分析结果表明，Foxp3的表达不是影响乳腺癌5年生存率的独立预后指标。结论：乳腺癌组织高表达Foxp3蛋白可作为一个潜在的乳腺癌生物标记物，但不是一个独立的预后指标。

Objective:To investigate the infiltration of Foxp3+ cells and expression of Foxp3 protein in breast carcinoma tissues and their clinical significance. Methods: One hundred and sixty breast carcinoma and 22 paracancerous tissues (who had been diagnosed in Shanghai Huangpu Center Hospital from Jan. 2005 to Dec. 2005) were included in present study. The infiltration of Foxp3+ cells and expression of Foxp3 protein in breast carcinoma and paracancerous tissues were detected by immunohistochemistry, and their relationships to clinicopathologic features and expression of ER,PR,P53,Bcl2,HER2 in breast carcinoma were analyzed. Results: Both positive rate of Foxp3+ cell infiltration in mesenchyma tissues and Foxp3 protein in parenchyma tissues were significantly higher than those in normal paracancerous tissues (P<0.05), but there was no correlation between the Foxp3+ cell infiltration and Foxp3 protein expression in breast carcinoma tissues (P>0.05). Foxp3+ cell infiltration in mesenchyma tissues was positively correlated to lymph node metastasis, histological grade and P53 overexpression (P<0.05); Foxp3 protein expression in parenchyma tissues was positively correlated to lymph node metastasis, histological grade, pTNM stage and P53 overexpression (P<005), but not to overexpression of ER, PR, HER2 and Bcl2 (P>0.05). Univariate survival analysis indicated that Foxp3 expression in breast carcinoma was associated with 5year overall survival rate, while multivariate analysis indicated that Foxp3 expression was not an independent prognostic factor for 5year overall survival rate in breast carcinoma (P>005). Conclusion: Foxp3 protein is overexpressed in breast carcinoma tissues, which might be a potential biomarker but not an independent prognostic factor in breast carcinoma.