目的：探讨人骨髓间充质干细胞（human bone marrow-derived mesenchymal stem cell，hBMSC）对胃癌细胞的趋化性，为将MSC研发成为胃癌基因治疗载体提供实验依据。 方法： 骨髓培养法分离培养hBMSC并进行流式细胞术鉴定。Transwell实验检测hBMSC对胃癌MKN45细胞和人成纤维细胞（human fibroblast，hFB）的趋化能力。建立MKN45细胞裸鼠移植瘤模型，感染Lenti-EGFP（MOI为50）的hBMSC或hFB细胞经尾静脉注射入荷瘤小鼠，荧光显微镜下观察移植瘤组织及各脏器GFP的表达。 结果： 培养第3代的hBMSC细胞CD44、CD105阳性率为（96.7±1.84）%、（98.1±0.95）%，而CD34、CD45表达阴性。hBMSC细胞向MKN45细胞的趋化能力明显强于胃上皮细胞GES-1组及空白对照细胞\[（239.5±54.3) vs (43.57±4.6)、(37.3±4.7)个，P<0.01\]，且hBMSC向MKN45细胞的趋化能力明显强于hFB细胞向MKN45细胞的趋化能力\[（239.5±54.3) vs (27.7±16.7)，P<0.01\]。与hFB相比，hBMSC对胃癌移植瘤组织具有明显趋向性；hBMSC组移植瘤组织内可见GFP表达，移植瘤小鼠部分肝脏（20%）及肺脏（20%）有GFP表达，但较移植瘤组织内GFP表达率和强度均低（P<005）。 结论： hBMSC在体内外对胃癌细胞均有特异性趋化作用，有望研发为胃癌基因治疗的良好载体。

Objective :To investigate the tropism of human bone-derived mesenchymal stem cells (hBMSCs) to gastric cancer cells in vitro and in vivo and to provide evidence for MSC as the vehicle for gene therapy of gastric cancer. Methods: hBMSCs were isolated and cultured using bone marrow plating method, and identified by flow cytometry. Migration abilities of hBMSCs to gastric cancer MKN45 cells and human fibroblast (hFB) cells were examined by Transwell assay. the tumor nude mice model transplanted by MKN45 cells was established. And Lenti-EGFP infected-hBMSC or-hFB (MOI=50) cells were subcutaneously injected into tumor-bearing mice, then GFP expression in transplanted tumors and different organs were observed under a fluorescence microscope. Results: The positive expression rates of CD44 and CD105 in the third passage hBMSC were (96.7±1.84)% and (98.1±0.95)% respectively, while CD34 and CD45 were negatively expressed. Chemotaxis of hBMSC to MKN45 cells was significantly higher than that to gastric epithelial GES-1 cells (239.5±54.3 vs 43.57±4.6, 37.3±4.7; P<0.01), and chemotaxis of hBMSC to MKN45 cells was significantly higher than that of hFB cells (P>0.05). hBMSC migrating to MKN45 were significantly higher than hFB (239.5±54.3 vs 27.7±16.7, P<0.01). Compared with hFB cells, hBMSC showed obvious chemotaxis to gastric transplanted tumor tissues; in hBMSC group, GFP was highly expressed in transplanted tumor tissues, as well as in liver (20%) and lung tissues (20%), but the later were significantly lower than that in transplanted tumor tissues (P<005). Conclusion: The chemotaxis of hBMSC to gastric cancer is obvious and specific, and hBMSC may be a good vehicle for gene therapy of gastric cancer.

国家自然科学基金资助项目（No. 81060198）；江西省教育厅项目（No. GJJ09429）；江西省科技厅项目（No. 2010BSA12800）