目的：探讨瘦素（leptin）对人脑胶质瘤U87MG细胞迁移及侵袭能力的影响及其机制。方法：Leptin处理U87MG细胞，采用细胞划痕实验检测U87MG细胞的迁移能力，Transwell实验检测U87MG细胞的侵袭能力，RT-PCR及Western blotting法检测U87MG细胞中MMP-2及MMP-9 mRNA和蛋白的表达。结果：Leptin明显促进U87MG细胞迁移能力\[（152.42±3.29）vs（83.24±2.61）μm，P<0.05\]和侵袭能力\[（31.78±5.04）vs（17.03±2.41）个细胞，P<0.05\]，leptin能显著上调U87MG细胞中MMP-2、MMP-9 mRNA \[（0.76±0.04）vs（0.35±0.02），（0.84±0.02）vs（0.41±0.06）；均P<0.05\]及蛋白\[（079±0.03）vs（0.23±0.01），（0.81±0.05）vs（0.39±0.03）；均P<0.05\]的表达。MMP抑制剂GM6001（10 μmol/ml)可以逆转leptin对U87MG细胞迁移\[（82.05±2.98）vs（81.76±3.25）μm，P>0.05\]和侵袭能力\[（19.23±2.46）vs（18.02±198）个细胞，P>0.05\]的影响。结论：Leptin可以促进人脑胶质瘤U87MG细胞的侵袭及迁移，其机制可能与上调MMP-2、MMP-9的表达有关。

Objective: To investigate the effect of leptin on the migration and invasion of human glioma U87MG cells, and explore its molecule mechanism. Methods: U87MG cells were treated with leptin, the cell migration was analyzed by cell scratch assay, cell invasion was detected by Transwell, and mRNA and protein expressions of MMP-2 and MMP-9 in U87MG cells were observed by RT-PCR and Western blotting assay. Results: Leptin significantly promoted migration (\[152.42±3.29\] vs \[83.24±2.61\] μm, P<0.05), and invasion (\[31.78±5.04\] vs \[17.03±2.41\]/field, P<0.05) of U87MG cells; the mRNA and protein expressions of MMP-2 and MMP-9 were significantly upregulated by leptin in U87MG cells (mRNA: \[0.76±0.04\] vs \[0.35±0.02\], \[0.84±0.02\] vs \[0.41±0.06\], P<0.05; protein: \[079±0.03\] vs \[0.23±0.01\], \[0.81±0.05\] vs \[0.39±0.03\], P<0.05); MMP inhibitor GM6001 reversed U87MG cell migration (\[82.05±2.98\] vs \[81.76±3.25\] μm, P>0.05\] and invasion (\[19.23±2.46\] vs \[18.02±1.98\]/field, P<0.05) induced by leptin. Conclusion: Leptin can promote the invasion and migration of human glioma U87MG cells, which might related with the upregulation of MMP-2 and MMP-9.

国家自然科学基金资助项目（No. 81101906)；上海市科委基金资助项目（No. 8411953600)