目的：检测TFIIB相关因子2（TFIIB-related factor 2，BRF2）基因在人食管鳞状细胞组织、癌旁组织及正常食管组织中的表达，分析BRF2在食管鳞状细胞癌发生、发展及预后中的意义。方法：选取2007年1月至2008年1月在山东大学齐鲁医院胸外科行手术治疗的食管鳞状细胞患者74例，应用RT-PCR和免疫组化方法检测食管鳞状细胞组织、癌旁组织和正常食管组织中BRF2 mRNA和蛋白表达水平。结果：食管鳞状细胞癌及其癌旁组织中BRF2 mRNA表达水平明显高于正常食管组织。食管鳞状细胞组织、癌旁组织和正常食管组织中BRF2蛋白表达阳性率分别为54.5%、32.5%和7.5%，癌组织、癌旁组织中BRF2蛋白的阳性率均显著高于正常食管组织（P<0.05）。随着食管鳞状细胞分化程度升高，BRF2蛋白阳性率显著下降，Ⅲ期和Ⅳ期食管鳞状细胞组织中BRF2蛋白阳性率明显高于Ⅰ期和Ⅱ期（72.7%，73.3% vs 35.7%，34.8%， P<0.05），且生存3年以下的食管鳞状细胞癌患者BRF2表达明显高于3年以上者（69.2% vs 38.2%，P<0.05），吸烟患者预后BRF2蛋白阳性率明显高于非吸烟患者（61.1% vs 30.4%，P<0.05）。结论：食管鳞状细胞癌组织高表达BRF2蛋白，BRF2 mRNA和蛋白与患者不良预后相关，可能作为食管鳞状细胞预后判断的参考指标之一。

Objective:To investigate the expression of TFIIB-related factor 2 (BRF2) in human esophageal squamous cell carcinoma tissues, adjacent esophageal cell carcinoma tissues and normal esophageal tissues, so as to analyze the role of BRF2 in the development and progression of esophageal squamous cell carcinoma. Methods: Samples were obtained from 74 esophageal squamous cell carcinoma patients who had been surgically treated during Jan. 2007 to Jan. 2008 in Qilu Hospital of Shandong University. Immunohistochemisty and RT-PCR were applied to detect BRF2 expression in esophageal squamous cell carcinoma tissues, adjacent esophageal squamous cell carcinoma tissues and normal esophageal tissues. Results: The expression of BRF2 mRNA in esophageal squamous cell carcinoma and adjacent esophageal squamous cell carcinoma tissues was significantly higher than that in normal esophageal tissues. The positive expression rates of BRF2 protein in esophageal squamous cell carcinoma, adjacent esophageal squamous cell carcinoma and normal esophageal tissues were 54.55%, 32.5% and 7.5%, respectively, with a rate in esophageal squamous cell carcinoma and adjacent esophageal squamous cell carcinoma tissues higher than that in normal esophageal tissues (P<0.05). The BRF2 positive expression rate in esophageal squamous cell carcinoma tissues was decreased with the increase in differentiation grade of esophageal squamous cell carcinoma (P<0.05), and the expression rate in grade Ⅲ and Ⅳ of esophageal squamous cell carcinoma significantly higher than that in gradeⅠ and Ⅱ ( 72.7%, 73.3% vs 35.7%, 34.8%， P<0.05). The BRF2 expression rate in the patients who survived less than 3 years was markedly higher than that in the patients who survived longer than 3 years (69.2% vs 38.2%, P<0.05); and smoking patients had a markedly higher expression rate than non-smokers (61.1% vs 30.4%, P<0.05). Conclusion: mRNA and protein of BRF2 gene are highly expressed in esophageal squamous cell carcinoma, which is correlated with poor prognosis of patients, and may be used in evaluating the prognosis of esophageal carcinoma patients.

国家自然科学基金资助项目（No. 30571844）