目的：研究Toll样受体7（Toll-like receptor 7，TLR7）mRNA及TLR9mRNA在慢性粒细胞白血病（chronic myeloid leukemia，CML）患者外周血浆细胞样树突状细胞（plasmacytoid dendritic cell，pDC）内的表达及pDC分泌干扰素-α（interferon-α，IFN-α）的能力。方法：收集兰州大学第二医院血液科2010年11月至2011年7月间收治的30例CML患者（初诊未治组15例，缓解组15例）和体检中心的15例健康对照者，运用免疫磁珠法分选外周血pDC，利用real time-PCR检测pDC内TLR7及TLR9 mRNA表达水平。CpG ODN 2216刺激pDC 24 h后，ELISA检测上清液中IFN-α水平。结果：初诊组CML患者外周血pDC内TLR7 mRNA表达水平显著低于缓解组\[（0.34±0.11） vs （0.93±0.21），P<0.05\]，初诊CML患者TLR9 mRNA表达水平显著低于缓解组\[（0.44±0.15） vs （0.94±0.18），P<0.05\]。CpG ODN 2216刺激后，初诊组pDC产生的IFN-α明显低于缓解组及健康对照组\[（408.61±77.11）vs（611.39±84.86）、（651.67±93.39）ng/L，P<0.05\]。结论：CML患者pDC内TLR7和TLR9 mRNA明显降低可能是pDC功能缺陷的主要原因，提示TLR7和TLR9可能参与CML的发病。

Objective: To investigate the expression of Toll-like receptor 7 (TLR7) mRNA and TLR9 mRNA in the peripheral blood plasmacytoid dendritic cells (pDCs) of patients with chronic myeloid leukemia (CML), and the interferon-α (IFN-α) secretion by pDCs. Methods: pDCs were sorted by immune magnetic beads of the patients with CML (n=30, including 15 CML untreated and 15 CML remission) from the Department of Hematology of Second Affiliated Hospital of Lanzhou University from November 2010 to July 2011 and healthy controls (n=15) from Physical Examination Center. The mRNA expressions of TLR7 and TLR9 in pDCs were measured with real time-PCR; pDCs were stimulated by CpG ODN 2216 for 24 h, and IFN-α in the supernatant was measured using ELISA. Results: The mRNA expression of TLR7 was significantly reduced in the untreated CML group (0.34±0.11) than that in the remission CML group (0.93±021, P<005) and the healthy control group (P<0.05); The mRNA expression of TLR9 was significantly reduced in the untreated CML group (0.44±0.15) than that in the remission group (0.94±0.18, P<0.05) and the healthy control group (P<0.05); IFN-α production of pDCs was significantly reduced in the untreated CML group than that in the remission group and the healthy control group (\[408.61±77.11\] vs \[611.39±84.86\], \[651.67±93.39\] ng/L, P<0.05). Conclusion:The mRNA expressions of TLR7 and TLR9are significantly reduced in CML patients, which may be the main reason of function defects of pDCs, indicating that TLR7 and TLR9 may be involved in the pathogenesis of CML.

甘肃省科技支撑计划资助项目（No. 0804NKCA115）