目的：研究姜黄素对人结肠癌SW480细胞增殖以及survivin、caspase-3和p-Akt表达的影响。方法：用不同浓度姜黄素（5~40 μmol/L）作用SW480细胞24、48和72 h，MTT法检测姜黄素对SW480细胞生长的抑制作用，Western blotting法检测SW480细胞中survivin、caspase-3和p-Akt蛋白的表达。结果：姜黄素以剂量（5~40 μmol/L)和时间(24~72 h)依赖的方式抑制SW480细胞的增殖（P<0.01），40 μmol/L姜黄素作用72 h对SW480细胞生长的抑制率可达（75.86±3.93）%。姜黄素抑制SW480细胞中p-Akt、survivin蛋白的表达、促进caspase-3蛋白的表达，均呈时间和剂量依赖性；40 μmol/L姜黄素作用SW480细胞48 h后 p-Akt和survivin蛋白表达显著减少\[（0.204±0.025) vs (0.367±0.035)，P<0.01；(0.208±0.014) vs (0385±0.034)，P<0.01\]，caspase-3蛋白表达显著增加\[（0.371±0.028) vs (0.127±0.023)，P<0.01\]。结论：姜黄素抑制SW480细胞增殖，其机制可能与抑制Akt磷酸化、下调survivin和上调caspase-3蛋白的表达有关。

Objective: To study the effect of curcumin on proliferation and expression ofsurvivin, caspase-3 and p-Akt in colon cancer SW480 cells.Methods:SW480 cells were treated with different concentrations of curcumin (5-40 μmol/L) for 24, 48 and 72 h. The inhibitory effect of curcumin on SW480 cells was detected by MTT. The expressions of survivin, caspase-3 and p-Akt protein were analyzed by Western blotting. Results: Curcumin decreased the proliferation of SW480 cells in a dose- and time-dependent manner (P<0.01), with an inhibitory rate of (75.86±3.93)% after 40 μmol/L curcumin treatment for 72 h. Curcumin decreased the expressions of p-Akt and survivin, and increased the expression of caspase-3 protein in a dose- and time-dependent manner (P<0.01). Treated with curcumin (40 μmol/L) for 48 h, the expressions of p-Akt and survivin protein were decreased significantly (\[0.204±0.025\] vs \[0.367±0.035\], P<0.01; \[0.208±0.014\] vs \[0.385±0.034\], P<0.01), and the expression of caspase-3 protein was increased significantly (\[0.371±0.028\] vs \[0.127±0.023\], P<0.01). Conclusion: Cureumin inhibits the proliferation of SW480 cells, which may be related to a decrease in phosphorylation of Akt, down- regulation of survivin protein expression and up-regulation of caspase-3 protein expression.

2009年浙江省大学生科技创新活动计划项目