目的：研究G蛋白偶联受体（G protein-coupled receptor，GPR）54基因的表达与骨肉瘤生物学行为和临床预后的关系。方法:应用RT-PCR检测3种人骨肉瘤细胞（MG-63、Saos-2、U-2 OS细胞）和正常人成骨hF-OB1.19细胞中GPR54 mRNA的表达情况，免疫组织化学技术检测44例骨肉瘤和12例骨软骨瘤组织中GPR54蛋白的表达情况，应用Transwell 侵袭实验比较不同骨肉瘤细胞的侵袭、迁移能力。结果：在人骨肉瘤MG-63、Saos-2、U-2 OS细胞和正常人成骨hF-OB1.19细胞中，GPR54 mRNA表达水平均较高，各组细胞间差别无统计学意义\[（0.87±0.05)、(0.88±0.07)、(0.88±0.08)、(0.86±0.05)，P>005\]。骨肉瘤MG-63、Saos-2、U-2 OS细胞侵袭、迁移细胞数逐渐降低\[(61.00±7.15) vs (32.40±5.36)、(18.10±321) 个，P<005或P<0.01\]。人骨肉瘤组织中GPR54蛋白表达阳性率显著高于骨软骨瘤组织(81.82% vs 41.67%，P<0.05)。GPR54蛋白阳性表达的骨肉瘤患者平均生存期明显短于GPR54蛋白阴性表达的患者\[(27.39±6.05) vs (40.33±4.88)月，P<005\]。结论：GPR54基因在骨肉瘤组织中的表达阳性率增高，且与患者临床生存期呈负相关。

Objective:To explore the relationship between the expression of G protein-coupled receptor (GPR)54 gene and the biologic behavior or prognosis of ostosarcoma patients.Methods: The expression levels of GPR54 mRNA in 3 types of osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS cells) and one normal osteoblast cell line hF-OB 1.19 were examined using RT-PCR. The expression levels of GPR54 protein in 44 osteosarcoma and 12 benign osteochondroma control paraffin specimen sections were detected by immunohistochemistry. Transwell assay was used to detect the migration and invasion abilities of the 3 types of osteosarcoma cell lines. Results: GPR54mRNA was strongly expressed in all the 3 types of osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS) and the normal osteoblast cell line (\[0.87±0.05\] vs \[088±0.07\], \[0.88±0.08\], \[0.86±0.05\], P＞0.05), but no significant difference existed between each other. Transwell assay displayed a gradually decreased aggressive phenomenon in osteosarcoma cell lines MG-63, Saos-2, U-2 OS (\[18.10±3.21\] vs \[32.40±5.36\], \[61.00±7.15\], P<0.05). The positive expression rate of GPR54 was significantly higher in osteosarcoma than in benign osteochondroma control (81.82% vs 41.67%, P<0.05). Significantly shorter average survival time was displayed in the patients with GPR54 protein positive expression than the in those with GPR54 protein negative expression (\[27.39±6.05\] months vs \[40.33±4.88\] months, P<0.05). Conclusion: The positive expression rate of GPR54 gene is higher in osteosarcoma patients than in benign osteochondroma patients, which might be associated with short survival time in osteosarcoma patients.

卫生部科学研究基金-福建卫生教育联合攻关计划（No. WKJ2008-2-042）