目的：检测子宫内膜癌（endometrial carcinoma，EC）组织中RECK（reversion-inducing cysteine-rich protein with Kazal motifs）mRNA和基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）mRNA的表达情况，探讨两者在子宫内膜癌中的临床意义。方法：选取2009年3月至2010年11月在滨州医学院附属医院妇科接受手术的子宫内膜癌患者组织标本42例，应用定量PCR法检测RECK mRNA及MMP-9 mRNA在子宫内膜癌中的表达情况，分析其相关性和临床意义。结果：在正常增生期子宫内膜、子宫内膜不典型增生、子宫内膜癌组织中RECK mRNA表达水平依次降低\[（6.30±0.34)、(4.29±0.36)、(0.24±018)，F=427.35，P<0.05\]，MMP-9 mRNA依次升高\[(0.08±0.82)、(5.04±0.30)、(6.22±0.32)，F=1117.52，P<0.05\]，RECK和MMP-9 mRNA的表达与子宫内膜癌临床分期、分化程度、淋巴转移关系密切（P<0.05），在子宫内膜癌中，MMP-9 mRNA和RECK mRNA之间存在负相关（r=-0.478，P<0.01）。结论：定量PCR检测RECK mRNA和MMP-9 mRNA对子宫内膜癌早期诊断及预测癌前病变风险有一定参考价值。

Objective: To investigate the expressions of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) mRNA and matrix metalloproteinase-9 (MMP-9) mRNA in endometrial carcinoma and explore their clinical significance. Methods: Forty-two endometrial carcinoma samples were obtained from gynecological surgery in the Affiliated Hospital of Binzhou Medical University (Mar. 2009 to Dec. 2010). Real-time quantitative PCR was used to assess the expressions of RECK and MMP-9 mRNA in endometrial carcinoma, and their relationship with clinic pathological features of endometrial carcinoma was analyzed. Results: The expression level of RECK mRNA was progressively decreased from the normal to invasive carcinoma (\[6.30±0.34\], \[4.29±0.36\], \[0.24±0.18\]，F=427.35, P<0.05）, while MMP-9 mRNA progressively increased (\[0.08±0.82\], \[5.04±0.30\], \[6.22±0.32\]，F=1117. 52, P<0.05). Both expressions of RECK and MMP-9 mRNA were correlated with TNM stage, histological grade and lymph node metastasis of endometrial carcinoma (P<0.05). There existed a significantly negative correlation between the expressions of RECK mRNA and MMP-9 mRNA in endometrial carcinoma (r=-0.832, P<0.01). Conclusion: Combined detection of RECK mRNA and MMP-9 mRNA by quantitative PCR has a certain value for predicting risk of early diagnosis of endometrial carcinoma and recancerous lesions.

山东省保健医学科研项目资助（No. 2007BZ20）