目的：构建携带钙网蛋白（calreticulin，CRT）基因和黑素瘤抗原基因-A3（melanoma antigen gene-A3， MAGE-A3）的重组腺病毒载体Ad-CRT/MAGE-A3，观察其联合表柔比星对人乳腺癌细胞MDA-MB-231增殖及侵袭的抑制作用。 方法： 构建Ad-CRT/MAGE-A3载体，感染MDA-MB-231细胞后，荧光显微镜观察其最适感染复数（multiplicity of infection，MOI）。分表柔比星、Ad-GFP、Ad-CRT/MAGE-A3、表柔比星+Ad-GFP、表柔比星+Ad-CRT/MAGE-A3共5组， MTT法检测各组MDA-MB-231细胞的增殖，Transwell实验检测各组MDA-MB-231细胞的侵袭力。 结果： 成功构建重组腺病毒载体Ad-CRT/MAGE-A3，其感染MDA-MB-231细胞的最适MOI为100。与其他各组相比，应用Ad-CRT/MAGE-A3联合表柔比星能明显抑制MDA-MB-231细胞的增殖\[(83.27±104)% vs（57.42±1.27）%，(43.26±0.95)%，(61.23±1.47)%，（55.38±1.62）%；P<0.05\]和侵袭\[（8.41±4.20）vs (14.62±5.33)，(107.66±3.35)，(100.60±4.42)，（104.20±2.60）；P<0.05\]。 结论： 腺病毒载体Ad-CRT/MAGE-A3能增强表柔比星抑制乳腺癌细胞MDA-MB-231增殖及侵袭的能力。

Objective : To construct a recombinant adenovirus for calreticulin (CRT)/melanoma antigen gene-A3 (MAGE-A3) and evaluate its inhibitory effect on breast cancer MDA-MB-231 cells. Methods: Ad-CRT/MAGE-A3 vector was constructed and infected into MDA-MB-231 cells. The optimal multiplicity of infection (MOI) was obtained by fluorescence microscope method. There were five groups: epirubicin, Ad-GFP, Ad-CRT/MAGE-A3, epirubicin+Ad-GFP and epirubicin+Ad-CRT/MAGE-A3. The proliferation and invasion of MDA-MB-231 cells in different groups were detected by MTT and Transwell assays. Results: Recombinant adenovirus vector Ad-CRT/MAGE-A3 was constructed successfully, and its optimal MOI in MDA-MB-231 cells was 100. Ad-CRT/MAGE-A3 combined with epirubicin decreased the proliferation (\[83.27±1.04\]% vs \[57.42±1.27\]%, \[43.26±0.95\]%, \[61.23±1.47\]%, \[55.38±1.62\]%, P<0.05) and the invasion (\[8.41±4.20\] vs \[14.62±5.33\], \[107.66±3.35\], \[100.60±442\], \[104.20±260\], P<0.05) of MDA-MB-231 cells significantly. Conclusion: Recombinant adenovirus vector Ad-CRT/MAGE-A3 can enhance epirubicin to inhibit the proliferation and invasion of breast cancer MDA-MB-231 cells.

辽宁省自然科学基金资助项目（No. 20042073）；辽宁省科技计划项目(No. 2009225008-1)