目的：研究γ-synuclein在食管癌中的表达及其对人食管癌细胞株Eca-109侵袭力的影响。方法: 选取2010年1月至12月于河北医科大学第四医院食管癌患者组织标本30例，同时取10例正常食管组织作对照。采用免疫组化法观察食管癌组织中γ-synuclein的表达情况。采用脂质体法转染含γ-synuclein的重组质粒pcDNA3.0-γ-synuclein，应用RT-PCR检测转染后Eca-109细胞中γ-synuclein mRNA的表达，流式细胞术检测γ-synuclein蛋白的表达，利用Transwell实验检测转染后Eca-109细胞侵袭力的变化。 结果： 与正常食管组织相比，食管癌组织中γ-synuclein蛋白阳性表达率明显增强（83.3% vs 400%，P<0.05），Ⅰ-Ⅱ期食管癌患者γ-synuclein表达率明显低于Ⅲ-Ⅳ期（22.2% vs 76.2%，P<0.05）。pcDNA3.0-γ-synuclein质粒转染后，Eca-109细胞中γ-synuclein mRNA表达水平显著高于空质粒转染组和未转染组\[（1.10±0.03) vs (0.42±0.03)，(0.45±0.15)，P<0.01\]，蛋白表达水平也明显增高\[（1.05±0.061) vs (0.80±0.45)，(0.79±0.46)；P<0.05\]，穿膜细胞数明显增加\[（167±2.51) vs (65±2.60)，(70±2.50)；P<0.01\]。 结论: 食管癌组织高表达γ-synuclein，其表达上调能增强人食管癌Eca-109细胞的侵袭力，提示其在食管癌的转移和恶性发展中发挥重要作用。

Objective : To detect the expression of γ-synuclein in esophageal adenocarcinoma and evaluate its effect on invasion of esophageal carcinoma Eca-109 cells. Methods: SP immunohistochemical staining was used to examine the expression of γ-synuclein in 30 esophageal carcinoma specimens and 10 normal control specimens from January to December 2010 (Fourth Hospital of Hebei Medical University). The plasmid containing γ-synuclein (pcDNA3.0-γ-synuclein) was transfected into Eca-109 cells by lipofectamine. The expressions of γ-synuclein mRNA and protein were investigated by RT-PCR and flow cytometry, respectively. The invasion ability of transfected Eca-109 cells was dectected by Transwell assay. Results: The positive exppression rate of γ-synuclein was 83% in human esophageal carcinoma, which was significantly higher than that in normal esophageal tissues (P<0.05), and the positive rate of γ-synuclein in stage-Ⅱ esophageal carcinoma patients was significantly lower than that in stage Ⅲ-Ⅳ patients (22.2% vs 76.2%，P<0.05). By comparing transfected group in Eca-109 cells with empty plasmid group and control group, RT-PCR showed the expression level of γ-synuclein mRNA increased significantly (\[1.10±0.03\] vs \[0.42±0.03\]，\[0.45±0.15\]; P<0.01). Flow cytometry showed that the expression level of γ-synuclein protein increased significantly compared with the other two groups (\[1.05±0.06\] vs \[0.80±0.45\], \[0.79±0.46\]; P<0.05). Invasion through matrigel abilities of Eca-109 cells increased significantly (\[167±2.51\] vs \[65±2.60\], \[70±2.50\]; P<0.01). Conclusion: γ-synuclein is highly expressed in esophageal carcinoma tissues and can enhance the invasion of Eca-109 cells, which may play an important role in carcinogenesis progression and metastasis of esophageal carcinoma.

河北省自然科学基金资助项目(No.C2008000948)