目的：研究肿瘤仿血管通道在骨肉瘤裸鼠移植瘤组织中的结构特点及其在肿瘤微血管形成中的作用。方法：建立荷人骨肉瘤MG-63细胞裸鼠移植瘤和荷骨肉瘤UMR106细胞大鼠移植瘤模型，以CD34、CD147免疫荧光和GFP荧光法观察移植瘤组织中肿瘤仿血管通道的结构特点及其与肿瘤微血管的关系。结果：骨肉瘤MG-63细胞移植瘤和UMR106细胞移植瘤组织CD147染色均呈阳性，血管内皮细胞CD34染色均阳性。肿瘤仿血管通道主要位于移植瘤组织中心区域，主要由骨肉瘤细胞构成，是具有功能的类血管通道；肿瘤微血管主要位于移植瘤周边区域，主要由宿主组织的血管内皮细胞组成，是与肿瘤仿血管通道存在直接相通的管道样结构。结论：大鼠和裸鼠骨肉瘤细胞移植瘤组织中存在的肿瘤仿血管通道具有类血管功能，并与来源于宿主组织的肿瘤微血管相互联通，诱导肿瘤微血管侵入肿瘤内部生长。

Objective:To investigate the structure characteristics and roles of vasculogenic mimicry in the xenografts of human osteosarcoma cells in nude mice. Methods: The models of xenografts of MG-63 human osteosarcoma cells in nude mice and xenografts of UMR106 osteosarcoma cells in nude rats were constructed. The construction features of vasculogenic mimicry and their relationship with tumor microvascular in transplanted osteosarcoma were observed by CD34 and CD147 immunofluorescence staining and GFP staining. Results:CD147 was positive for MG-63-cell-transplanted osteosarcoma tissues and CD34 wsa positive for vascular endothelial cells. The construction of vasculogenic mimicry was observed in the central area of transplanted osteosarcoma, which was constituted mainly by osteosarcoma cells and had the function of the blood vessel. The construction of tumor microvascular was observed in the surrounding area of transplanted osteosarcoma, which was constituted mainly by vascular endothelial cells and connected to vasculogenic mimicry by certain pipeline sample structure. Conclusion:Vasculogenic mimicry in osteosarcoma tissues functions as the blood vessels and can be connected to tumor microvascular so that they can induce tumor microvascular infiltrating into internal tumors.

国家自然科学基金资助项目（No.30471760）